Analysis of novel proteins abundantly expressed in extremo-tolerant tardigrades.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KUNIEDA Takekazu 東京大学, 理学(系)研究科(研究院), 助教 (10463879)
• Co-Investigator(Renkei-kenkyūsha): KUBO Takeo 東京大学, 大学院理学系研究科, 教授 (10201469)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 緩歩動物 / 乾燥耐性 / 熱可溶性 / クマムシ

Research Abstract

Limno-terrestrial tardigrades can tolerate severe desiccation by entering an ametabolic dehydrated state called anhydrobiosis. Here, we focused and analyzed properties of novel proteins abundantly expressed in anhydrobiotc tardigrades as candidates involved in anhydrobiosis. A1 is the most abundantly expressed transcript and its protein products turned out to form an aggregated complex in a spiral shape. This aggregated structure could provide physical support to desiccating cells. We also identified a novel heat-soluble protein from less tolerant tardigrade species and showed the amount of heat-soluble proteins can correlate with tolerant ability of the species. Furthermore, as a candidate involved in protection of mitochondria against desiccation, novel mitochondrial proteins were identified.

Research Products

• [Journal Article] Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade (2012)
  • Author(s): Yamaguchi A, Tanaka S, Yamaguchi S, Kuwahara H, Takamura C, Imajoh-Ohmi S, Horikawa D. D, Toyoda A, Katayama T, Arakawa K, Fujiyama A, Kubo T and Kunieda T
  • Journal Title: PLoS ONE Volume: 7 Pages: e44209
  • Peer Reviewed
• [Presentation] ヨコヅナクマムシにおける新規ミトコンドリアタンパク質の同定 (2013)
  • Author(s): 田中冴、秦裕子、尾山大明、豊田敦、片山俊明、荒川和晴、藤山秋佐夫、久保健雄、國枝武和
  • Organizer: 細胞を創る研究会6.0
  • Place of Presentation: 山形・鶴岡
  • Year and Date: 20131114-15
• [Presentation] ヨコヅナクマムシにおける新規ミトコンドリアタンパク質の同定 (2013)
  • Author(s): 田中冴、秦裕子、尾山大明、豊田敦、片山俊明、荒川和晴、藤山秋佐夫、久保健雄、國枝武和
  • Organizer: 第14回極限環境生物学会
  • Place of Presentation: 神奈川・川崎
  • Year and Date: 20131026-27
• [Presentation] ヨコヅナクマムシにおける新規ミトコンドリアタンパク質の同定 (2013)
  • Author(s): 田中冴、秦裕子、尾山大明、豊田敦、片山俊明、荒川和晴、藤山秋佐夫、久保健雄、國枝武和
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 兵庫・神戸
  • Year and Date: 2013-12-04
• [Presentation] Identification of novel mitochondrial proteins from an anhydrobiotic tardigrade (2013)
  • Author(s): Tanaka S, Toyoda A, Katayama T, Arakawa K, Fujiyama A, Kubo T, and Kunieda T
  • Organizer: Dynamito 2013
  • Place of Presentation: Okinawa
  • Year and Date: 2013-11-01
• [Presentation] Genome annotations and transcriptomic analysis in Ramazzottius varieornatus (2012)
  • Author(s): Kunieda T, Arakawa K, Katayama T, Toyoda A, Kuwahara H, Horikawa DD, Suzuki Y, Abe K, Shin-I T, Ohishi K, Motoyama A, Aizu T, Kohara Y, Fujiyama A
  • Organizer: 12th International Symposium on Tardigrada
  • Place of Presentation: ポルト(ポルトガル)
  • Year and Date: 20120723-26
• [Presentation] Two families of novel abundant heat-soluble proteins in an anhydrobiotic tardigrade (2012)
  • Author(s): Tanaka S, Yamaguchi A, Yamaguchi S, Kuwahara H, Takamura C, Ohmi S, Horikawa DD, Toyoda A, Katayama T, Arakawa K, Fujiyama A, Kubo T, Kunieda T
  • Organizer: 12th International Symposium on Tardigrada
  • Place of Presentation: ポルト(ポルトガル)
  • Year and Date: 20120723-26
• [Presentation] ヨコヅナクマムシにおいて大量に発現する新規な熱可溶性タンパク質CAHS, SAHSの解析 (2012)
  • Author(s): 山口理美、田中冴、山口志保、久保健雄、國枝武和
  • Organizer: 第13回極限環境生物学会
  • Place of Presentation: 日本大学・東京
  • Year and Date: 2012-12-01
• [Presentation] 乾燥耐性の異なる複数のクマムシ種における熱可溶性タンパク質の解析 (2012)
  • Author(s): 伊藤麻紀子、鈴木忠、近藤裕子、秦裕子、尾山大明、久保健雄、國枝武和
  • Organizer: 第13回極限環境生物学会
  • Place of Presentation: 日本大学・東京
  • Year and Date: 2012-12-01
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/kuma/