2013 Fiscal Year Final Research Report
New NMR methods developed for structure determination of large proteins
| Project/Area Number |
24657074
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
KOJIMA Chojiro 大阪大学, たんぱく質研究所, 准教授 (50333563)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Toshimichi 大阪大学, 蛋白質研究所, 教授 (20242381)
MATSUKI Yoh 大阪大学, 蛋白質研究所, 助教 (70551498)
OHKI Izuru 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80418574)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | NMR / 構造生物学 / フロリゲン |
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| Research Abstract |
NMR structure can be determined for proteins with molecular weight less than 50 kDa, however, no structure for beyond 100 kDa. In this research, we have developed a new NMR method to analyze very large proteins. This method consists of sensitivity enhancement of 13C direct detection, and 13C assignment and analysis procedures employed for solid state NMR. These methods were applied to florigen receptor with molecular mass beyond 50 kDa.
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[Journal Article] Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac12014
Author(s)
Ken-ichi Kosami, Izuru Ohki, Kokoro Hayashi, Ryo Tabata, Sayaka Usugi, Tsutomu Kawasaki, Toshimichi Fujiwara, Atsushi Nakagawa, Ko Shimamoto, and Chojiro Kojima
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Journal Title
Acta Cryst
Volume: F70
Pages: 113-115
Peer Reviewed
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[Journal Article] The structure of metallo-DNA with consecutive T-Hg^<II>-T base-pairs explains positive entropy for the metallo-base-pair formation2014
Author(s)
Hiroshi Yamaguchi, JakubŠebera, Jiro Kondo, Shuji Oda, Tomoyuki Komuro, Takuya Kawamura, Takenori Dairaku, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Jaroslav V. Burda, Chojiro Kojima, Vladimír Sychrovský, and Yoshiyuki Tanaka
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Journal Title
Nucleic Acids Res.
Volume: 42
Pages: 4094-4099
Peer Reviewed
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[Journal Article] Site-specific isotope labeling of long RNA for structural and mechanistic studies2012
Author(s)
Ikumi Kawahara, Kaichiro Haruta, Yuta Ashihara, Daichi Yamanaka, Mituhiro Kuriyama, Naoko Toki, Yoshinori Kondo, Kenta Teruya, Junya Ishikawa, Hiroyuki Furuta, Yoshiya Ikawa, Chojiro Kojima and Yoshiyuki Tanaka
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Journal Title
Nucleic Acids Res
Volume: 40
Pages: e7
Peer Reviewed
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[Journal Article] 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen2011
Author(s)
Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Kyoko Furuita, Kokoro Hayashi, Tomoko Yanase, Midori Yamaguchi, Chika Nakashima, Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki, Chihiro Shimada, Atsushi Nakagawa, Chojiro Kojima and Ko Shimamoto
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Journal Title
Nature
Volume: 476
Pages: 332-335
Peer Reviewed
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[Presentation] Protein Modification Method for Studying Protein-Protein Interaction by NMR2011
Author(s)
Yoshikazu Hattori, Izuru Ohki, Kyoko Furuita, Takahisa Ikegami, Harumi Fukada, Masahiro Shirakawa, Kei-ichi Yokoyama, Ei-ichiro Suzuki, Toshimichi Fujiwara, Chojiro Kojima
Organizer
The 50^<th> Memorial Annual Meeting of the Nuclear Magnetic Resonance Society of Japan
Place of Presentation
Osanbashi Hall, Yokohama, Japan
Year and Date
20111115-18
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