2012 Fiscal Year Final Research Report

Experimental development a new cryo-holder suitable to electron cryo-tomography

Research Project

Project/Area Number	24657075
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Kyushu Institute of Technology
Principal Investigator	YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
Project Period (FY)	2012
Keywords	クライオ電子顕微鏡 / 構造生物学 / 電子顕微鏡法 / 試料ホルダー / 蛋白質構造解析
Research Abstract	The new cryo-holder suited to electron cryo-tomography was planed by using low-vibration cryo-pump and has been developed. Furthermore, conventional liquid-nitrogen-type cryo-holder was tested and successfully achieved a lower-temperature stage than conventional one by drawing vacuum with vacuum pumps. Hoses for vacuum drawing were important for less vibration of the stage and better hoses give the stable stage to obtain electro micrographs. Currently we are planning to develop a new holder by combining both techniques.