Time-resolved small-angle X-ray scattering of GUV deformed by F-BAR domain

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: NOBUTAKA Shimizu 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (20450934)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Masato 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (00342600) ; IGARASHI Noriyuki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 生体膜変形 / 時分割X線小角散乱 / ストップトフロー

Research Abstract

Some proteins transform an intracellular organelle and maintain structure. It is important to study those interactions in order to understand the structure and the function of the organelle. Then, a stopped-flow was installed in the small-angle X-ray scattering beamline at the Japanese synchrotron facility, Photon Factory, and the structural change of Giant unilamellar vesicle (GUV) interacted by the BAR domain protein was measured by time-resolve method. As a result, although it was very preliminary analysis, it was suggested the structure of GUV was changed with BAR domain in the space scale of around 125 angstrom within 210 seconds.

Research Products

• [Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling (2013)
  • Author(s): Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N.
  • Journal Title: J. Synchrotron Rad. Volume: 20 Pages: 869-874
  • Peer Reviewed
• [Journal Article] Refurbishing of Small-Angle X-ray Scattering Beamline, BL-6A at the Photon Factory (2013)
  • Author(s): Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
  • Journal Title: J. Phys. : Conf. Ser. Volume: 425 Pages: 202008
  • Peer Reviewed
• [Journal Article] New high-brilliance beamline BL-15A of the Photon Factory (2013)
  • Author(s): Igarashi, N., Shimizu, N., Koyama, A., Mori, T., Ohta, H., Niwa, Y., Nitani, H., Abe, H., Nomura, M., Shioya, T., Tsuchiya, K. and Ito, K.
  • Journal Title: J. Phys. : Conf. Ser. Volume: 425 Pages: 072016
  • Peer Reviewed
• [Journal Article] The binding mechanism of eIF2βwith its partner proteins, eIF5 and eIF2βε (2012)
  • Author(s): Gai, Z., Kitagawa, Y., Tanaka, Y., Shimizu, N., Komoda, K., Tanaka, I. and Yao M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 423 Pages: 515-519
  • Peer Reviewed
• [Presentation] PF BL-15A : 高輝度XAFS/XRF/SAXSビームライン (2014)
  • Author(s): 五十嵐教之、小山篤、森丈晴、上條亜衣、西條慎也、大田浩正、仁谷浩明、丹羽尉博、阿部仁、清水伸隆
  • Organizer: 第27回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: 広島国際会議場(ポスター)
  • Year and Date: 2014-01-11
• [Presentation] Refurbishment of SAXS beamlines at Photon Factory (2013)
  • Author(s): Nobutaka Shimizu
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館(口頭(企画講演))
  • Year and Date: 2013-10-28
• [Presentation] Improvement and Upgrade Plan of Small-Angle X-ray Scattering Beamlines at Photon Factory (2013)
  • Author(s): Shimizu, N., Mori, T., Ohta, H., Nagatani, Y., Saijyo, S., Kamijyo, A., Kosuge, T. and Igarashi, N.
  • Organizer: 11th International Conference on Biology and Synchrotron Radiation
  • Place of Presentation: Hamburg、Germany(ポスター)
  • Year and Date: 2013-09-09
• [Presentation] BL-15A : Semi-microbeam XAFS/XRF and High- brilliance SAXS beamline at Photon Factory (2013)
  • Author(s): Igarashi, N., Koyama, A., Mori, T., Ohta, H., Kamijo, A., Saijyo, S., Niwa, Y., Nitani, H., Abe, H. and Shimizu, N.
  • Organizer: 11th International Conference on Biology and Synchrotron Radiation
  • Place of Presentation: Hamburg、Germany(ポスター)
  • Year and Date: 2013-09-09
• [Presentation] Photon Factoryの小角散乱ビームラインの現状 (2013)
  • Author(s): 清水伸隆、五十嵐教之、森丈晴、大田浩正、永谷康子、小菅隆、伊藤健二
  • Organizer: 第26回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: 名古屋大学(口頭)
  • Year and Date: 2013-01-13
• [Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory (2012)
  • Author(s): Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
  • Organizer: 15th International Small-Angle Scattering conference (SAS2012)
  • Place of Presentation: Sydney、Australia(ポスター)
  • Year and Date: 2012-11-20
• [Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory (2012)
  • Author(s): Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学(ポスター)
  • Year and Date: 2012-09-24
• [Presentation] Development of Small-Angle X-ray Scattering Beamline, BL-6A at the Photon Factory (2012)
  • Author(s): Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
  • Organizer: 11th International Conference on Synchrotron Radiation Instrumentation (SRI2012)
  • Place of Presentation: Lyon, France(ポスター)
  • Year and Date: 2012-07-11