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2013 Fiscal Year Final Research Report

Prediction and screening of selective autophagy cargo in Saccharomyces cerevisiae

Research Project

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Project/Area Number 24657083
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Kuninori  東京大学, 新領域創成科学研究科, 准教授 (20373194)

Co-Investigator(Renkei-kenkyūsha) NODA Nobuo  微生物化学研究所, 主席研究員 (40396297)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords細胞内タンパク質分解 / オートファジー / 選択的オートファジー / オートファゴソーム / 出芽酵母
Research Abstract

Macroautophagy (autophagy) is a bulk protein-degradation system ubiquitously conserved in eukaryotic cells. During autophagy, cytoplasmic components are enclosed in a membrane compartment, called an autophagosome. In this study, we developed a method for monitoring intact autophagosomes ex vivo by detecting the fluorescence of GFP-fused aminopeptidase I, the best-characterized selective cargo of autophagosomes in Saccharomyces cerevisiae. A combination of LC-MS/MS with subsequent statistical analyses revealed a list of autophagosome cargo proteins. The methods we describe will be useful for analyzing the mechanisms and physiological significance of Atg11-independent selective autophagy.

  • Research Products

    (8 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae2014

    • Author(s)
      Kuninori Suzuki, Shingo Nakamura, Mayumi Morimoto, Kiyonaga Fujii, Nobuo N. Noda, Fuyuhiko Inagaki, Yoshinori Ohsumi
    • Journal Title

      PLoS One

      Volume: 9 Pages: e91651

    • DOI

      10.1371/journal.pone.0091651

    • Peer Reviewed
  • [Journal Article] Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae2013

    • Author(s)
      Kuninori Suzuki, Manami Akioka, Chika Kondo-Kakuta, Hayashi Yamamoto and Yoshinori Ohsumi
    • Journal Title

      J. Cell Sci.

      Volume: 126 Pages: 2534-2544

    • DOI

      10.1242/jcs.122960

    • Peer Reviewed
  • [Journal Article] Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae2013

    • Author(s)
      Risa Matsumoto, Kuninori Suzuki and Yoshikazu Ohya
    • Journal Title

      Protoplasma

      Volume: 250 Pages: 1283-1293

    • DOI

      10.1007/s00709-013-0510-2

    • Peer Reviewed
  • [Journal Article] Selective autophagy in budding yeast2013

    • Author(s)
      Kuninori Suzuki
    • Journal Title

      Cell Death Differ

      Volume: 20 Pages: 43-48

    • DOI

      10.1038/cdd.2012.73

    • Peer Reviewed
  • [Journal Article] Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg22012

    • Author(s)
      Takafumi Kobayashi, Kuninori Suzuki and Yoshinori Ohsumi
    • Journal Title

      FEBS Lett

      Volume: 586 Pages: 2473-2478

    • DOI

      10.1016/j.febslet.2012.06.008

    • Peer Reviewed
  • [Presentation] オートファジーによる被分解タンパク質の網羅的解析2013

    • Author(s)
      鈴木邦律
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      20130619-21
  • [Presentation] Fine mapping of現在これらのタンパク質がAPに選択的に取autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae2012

    • Author(s)
      鈴木邦律
    • Organizer
      第64回日本細胞生物学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      20120528-31
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

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Published: 2015-06-25  

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