2013 Fiscal Year Final Research Report
Analyses of the molecular interaction between epithelial organogenesis and immune system via specific antigens
| Project/Area Number |
24657086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
IZUTSU YUMI 新潟大学, 自然科学系, 准教授 (20301921)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 発生・分化 / 免疫学 / 蛋白質 / 発現制御 / 動物 |
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| Research Abstract |
We previously found a novel protein, named Ouro, as a candidate for immune antigens from amphibian. In this study, to clarify molecular mechanisms underlying the body plan of vertebrate animals, we focused on the functions of acquired immune system vs specific self-antigens, i.e., Ouro proteins. First, we examined immune cell response to the self-organ that expresses Ouro proteins. Using our transgenic Xenopus line, we found that immune T cells immediately migrated into Ouro expressing cells in the tadpole tail by using a live-cell-tracking. Second, we obtained polyclonal rat antisera against Ouro proteins. Third, using these antisera, we have tried the immunohistochemical analyses, and shown that a unique intracellular localization was seen when the Ouro proteins are recognized by immune cells.
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