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2013 Fiscal Year Final Research Report

Dynamic conformational changes that regulate the energy-converting unit

Research Project

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Project/Area Number 24657087
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionNagoya University

Principal Investigator

KOJIMA Seiji  名古屋大学, 理学(系)研究科(研究院), 准教授 (70420362)

Co-Investigator(Renkei-kenkyūsha) HOMMA Michio  名古屋大学, 大学院理学研究科, 教授 (50209342)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords細菌べん毛 / モーター / 膜タンパク質 / 構造変化
Research Abstract

The PomA/PomB complex, that functions as an energy-converting stator unit in the bacterial flagellar motor, becomes functional when installed into the appropriate place around the rotor. In the functional stator, it is believed that a conformational change in the periplasmic region of PomB is induced to anchor it and simultaneously to activate ion conduction activity of the stator. This study aims to detect such a conformational change using the fluorescent probe and the purified PomA/B complex reconstituted into the liposome. We are still on the way to establish the analyzing system, but we greatly improved the overproduction and purification procedure for the PomA/B complex, so that we could systematically screen conditions to reconstitute the functional stator complex to the liposome. We also succeeded in narrowing down the region where the conformational change occurs, by using the systematic disulfide bridge formation analysis.

  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (4 results) Presentation (7 results) (of which Invited: 3 results)

  • [Journal Article] Biophysical characterization of the C-terminal region of FliG, an essential rotor component of the Na^+ driven flagellar motor2014

    • Author(s)
      Gohara M, Kobayashi S, Abe-Yoshizumi R, Nonoyama N, Kojima S, Asami Y and Homma M
    • Journal Title

      J. Biochem

      Volume: 155 Pages: 83-89

  • [Journal Article] Construction of functional fragments of the cytoplasmic loop with the C-terminal region of PomA, a stator component of the Vibrio Na^+ driven flagellar motor2014

    • Author(s)
      Onoue Y, Abe-Yoshizumi R, Gohara M, Kobayashi S, Nishioka N, Kojima S, Homma M
    • Journal Title

      J Biochem

      Volume: 155 Pages: 207-216

  • [Journal Article] Contribution of many charged residues at the stator-rotor interface of the Na^+-driven flagellar motor to torque generation in Vibrio alginolyticus2014

    • Author(s)
      Takekawa N, Kojima S, Homma M
    • Journal Title

      J. Bacteriol

      Volume: 196 Pages: 1377-1385

  • [Journal Article] Intragenic suppressor of a plug deletion nonmotility mutation in PotB, a chimeric stator protein of sodium-driven flagella2012

    • Author(s)
      Zhu S, Homma M, Kojima S
    • Journal Title

      J. Bacteriol

      Volume: 194 Pages: 6728-6735

  • [Presentation] Na^+駆動型細菌べん毛モーター固定子の機能的集合と構造変化2014

    • Author(s)
      小嶋誠司
    • Organizer
      2013年度生物物理学会中部支部会
    • Place of Presentation
      岡崎カンファレンスセンター
    • Year and Date
      2014-03-06
  • [Presentation] Assembly-coupled activation mechanism of the Na^+-driven stator complex2014

    • Author(s)
      Seiji Kojima
    • Organizer
      Gordon Research Conference"Sensory Transduction in Microorganisms"
    • Place of Presentation
      Ventura Beach Mariott, Ventura, CA, USA
    • Year and Date
      2014-01-14
    • Invited
  • [Presentation] Structure-function analyses of the large conformational change required for the stator activation and assembly in the bacterial flagellar motor2013

    • Author(s)
      Seiji Kojima
    • Organizer
      8^<th> Asian Biophysics Association Symposium
    • Place of Presentation
      Ramada Plaza Hotel, Jeju, Korea
    • Year and Date
      20130526-29
  • [Presentation] Na^+駆動型べん毛モーター固定子タンパク質PomBの機能に重要なペリプラズム側構造変化の解析2013

    • Author(s)
      小嶋誠司
    • Organizer
      第77回日本生化学会中部支部会
    • Place of Presentation
      名古屋大学・坂田平田ホール
    • Year and Date
      2013-05-25
  • [Presentation] 細菌べん毛の構築と機能を制御するしくみ2013

    • Author(s)
      小嶋誠司
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ国際会議場
    • Year and Date
      2013-03-19
    • Invited
  • [Presentation] Structure and function of the periplasmic region of the bacterial flagellar stator protein PomB2012

    • Author(s)
      Seiji Kojima
    • Organizer
      Gordon Research Conference"Bacterial Cell Surfaces"
    • Place of Presentation
      Mount Snow Resort, West Dover, VT, USA
    • Year and Date
      20120624-29
  • [Presentation] 細菌べん毛運動マシナリー : 機能的固定子複合体の集合と活性化2012

    • Author(s)
      小嶋誠司
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-15
    • Invited

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Published: 2015-06-25  

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