2014 Fiscal Year Final Research Report
Analysis of the regulation-mechanism of intracellular dynamics of late endosome and lysosome by using a FRET biosensor
| Project/Area Number |
24657094
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | Gタンパク質 / シグナル伝達 / 分解経路 / リソソーム / FRET |
|---|
| Outline of Final Research Achievements |
Rab7 is known to be a key molecule which regulates dynamics of lysosomes, in which a variety of biomolecules and damaged organelles are destructed. In this study, I have developed a FRET sensor, Raichu-Rab7, which can visualize the spatiotemporal change of Rab7 activity in living cells. Raichu-Rabs7 has shown the existence of a significant variation in Rab7 activity of individual endosomes in steady-state COS-7 cells. Next, I investigated the mechanism regulating Rab7 activity during the process from late endosomes to lysosomes in steady-state cells and macropinocytosis in EGF-stimulated COS-7 cells. Based on these studies, I have concluded that Rab7 on late endosomes is activated by Mon1/Ccz1 complex and plays an essential role in the fusion between late endosome and lysosome; on the contrary, Rab7 activation on lysosomes is independent of Mon1/Ccz1 and active Rab7 on lysosomes is implicated in the perinuclear accumulation of lysosomes.
|
| Free Research Field |
分子神経科学
|
