2013 Fiscal Year Final Research Report
Identification of nascent polypeptides with novel functions
| Project/Area Number |
24657095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
CHIBA Shinobu 京都産業大学, 総合生命科学部, 助教 (20523517)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 蛋白質膜組込 / 翻訳アレスト |
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| Research Abstract |
We tried to identify novel nascent polypeptide chains that function during being synthesized. In the course of our screening, we are realizing that vast majority of cellular proteins undergoes transient pauses during translation elongation. These results are challenging our conventional view of translation elongation, while, at the same time, they are displaying too many candidates for novel regulatory nascent chains. Genetic modification of previously-identified regulatory nascent chain allowed to invent novel sensors. Original native sensors also found to function a powerful research tool to investigate molecular mechanisms of a protein membrane insertion machinery.
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