2013 Fiscal Year Final Research Report
Formation mechanism of proteasome granules
| Project/Area Number |
24657097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SAEKI Yasushi 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 副参事研究員 (80462779)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞内タンパク質分解 / ユビキチン / プロテアソーム / 酵母 |
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| Research Abstract |
The proteasome is an ATP-dependent protease complex responsible for targeted protein degradation in eukaryotic cells. To identify factors that affect the proteasome dynamics, we screened a yeast mutant library and found that the proteasome formed a large cytoplasmic granule in almost all the mitochondrial mutants. We found that lowered cellular ATP levels induce the granule formation of the proteasome even in the wild-type cells. Importantly, the granule formation of the proteasome is conserved in yeast and humans. Furthermore, the purified proteasomes formed fibrous aggregated structures by ATP depletion. Taken together, these results suggest that the proteasome itself senses cellular ATP levels and autonomously forms reversible granule structure in responding to cellular environments.
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[Journal Article] Structural basis of proteasome formation controlled by an assembly chaperone Nas22014
Author(s)
Satoh, T., Saeki, Y., Hiromoto, T., Wang, Y., Uekusa, Y., Yagi, H., Yoshihara, H., Yagi-Utsumi, M., Mizushima, T., Tanaka, K. and Kato, K.
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Journal Title
Structure
Volume: 22
Pages: 731-743
DOI
Peer Reviewed
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[Journal Article] Quantitative live-cell imaging reveals spatio-temporal dynamics and cytoplasmic assembly of the 26S proteasome2014
Author(s)
Pack, CG., Yukii, H., Toh-e, A., Kudo, T., Tsuchiya, H., Kaiho, A., Sakata, E., Murata, S., Sako, Y., Baumeister, W., Tanaka, K., and Saeki, Y.
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Journal Title
Nature Commun.
Volume: 5
Pages: 3396
DOI
Peer Reviewed
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[Journal Article] Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p2012
Author(s)
Takagi, K., Kim, S., Yukii, H., Ueno, M., Morishita, R., Endo, Y., Kato, K., Tanaka, K., Saeki, Y., and Mizushima, T.
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 12172-12182
DOI
Peer Reviewed
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[Journal Article] Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy2012
Author(s)
Sakata, E., Bohn, S., Mihalache, O., Kiss, P., Beck, B., Nagy, I., Nickell, S., Tanaka, K., Saeki, Y., Förster, F., and Baumeister, W.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 109
Pages: 1479-1484
DOI
Peer Reviewed
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