2013 Fiscal Year Final Research Report
Single molecule observation of protein translocation through translocon
| Project/Area Number |
24657106
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
SAKAGUCHI MASAO 兵庫県立大学, 生命理学研究科, 教授 (30205736)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | シグナル配列 / 生体膜 / オルガネラ |
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| Research Abstract |
Protein translocation through translocon on the endoplasmic reticulum in the eukaryotic cells is one of the most critical bases to maintain the life of the cells. The challenge in this project was to establish the experimental system by which the movement of the polypeptide chain through translocon can be observed at the single molecule level. We have set up the system in which we can observe protein translocation synchronously across the membrane and found that only 2-3 positively charged amino acid residues slow down the movement through translocon.
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[Journal Article] Stop-and-move of a marginally hydrophobic segment translocating across the endoplasmic reticulum membrane2013
Author(s)
Onishi, Y., Yamagishi, M., Imai, K., Fujita, H., Kida, Y., and Sakaguchi, M.
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Journal Title
J. Mol. Biol.
Volume: 425
Pages: 3205-3216
DOI
Peer Reviewed
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[Journal Article] Tail-extension following the termination codon is critical for release of the nascent chain from membrane-bound ribosomes in a reticulocyte lysate cell-free system2013
Author(s)
Takahara, M., Sakaue, H., Onishi, Y., Yamagishi, M., Kida, Y., and Sakaguchi, M.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 430
Pages: 567-572
DOI
Peer Reviewed
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