2014 Fiscal Year Final Research Report
Vibrational coherence investigation of protein fluctuations for dynamic allostery
| Project/Area Number |
24657107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KUBO Minoru 独立行政法人理化学研究所, 放射光科学総合研究センター, 専任研究員 (90392878)
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| Research Collaborator |
NAGATOMO Shigenori 筑波大学, 数理物質系化学域, 講師 (80373190)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 揺らぎ / 振動コヒーレンス / ヘモグロビン / タンパク質 / 低振動 / フェムト秒レーザー |
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| Outline of Final Research Achievements |
Vibrational coherence spectroscopy was applied to study the correlation between fluctuation of heme and its O2 affinity in hemoglobin (Hb). Damping times of low-frequency coherences (<200 cm-1) of heme, including doming mode coherence, were compared between 5 deoxyHbs with different O2 affinities, regulated by heterotropic effectors. The results showed that the damping times were not correlated with the O2 affinities. Instead, it was confirmed that the Fe-His stretching frequencies were correlated with the O2 affinities. Thus, this study supports Perutz model for the Hb function, although the role of fluctuation of the O2 channel remains to be examined.
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| Free Research Field |
生物物理学
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