2013 Fiscal Year Final Research Report
Control of protein localization and activity using photoreceptors
| Project/Area Number |
24657109
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 光生物 / バイオイメージング / 生体高分子構造・機能 / 生物工学 |
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| Research Abstract |
We have developed a system to control the light-swichable gene expression by using photoreceptive molecules. In order to polish this system toward application as a novel biological tool, we further characterize CRY photoreception and interaction proteins in this study. We have detected the positive fluorescent signals on the plasma membrane of mammalian cultured cells. We found relatively high expression levels of chicken cryptochrome in a constitutive expression system using budding yeast. We also succeeded in the purification of the cryptochrome protein having the chromophore FAD, investigation of the spectral properties, and detection of in vitro structural changes induced by light.
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Research Products
(23 results)
