Mechanism of how core transcription factors affect the fate of mRNA

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657119
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Yokohama City University
• Principal Investigator: KOKUBO Tetsuro 横浜市立大学, 生命医科学研究科, 教授 (10271587)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: transcription / TFIID / SAGA / mRNA / cyclin / gene regulation / cell morphogenesis / GTF

Outline of Final Research Achievements

TFIID, the largest GTF composed of TBP and 14 TAFs, and its related complex SAGA play critical roles in transcriptional activation by regulating TBP-DNA interactions. Previously,we suggested an intriguing possibility that CLN2 mRNA generated by TFIID or SAGA (designated as CLN2 mRNA[TFIID] or [SAGA], respectively) could produce two types of Cln2p that carry distinct functions.
Here we demonstrate that TAND (Taf1p N-terminal domain) of TFIID may determine the production ratio between CLN2 mRNA[TFIID] and [SAGA], and also that the latter mRNA may be specifically regulated by Ssd1p and RAM (Regulation of Ace2p activity and cellular Morphogenesis) signaling pathway at the translational level. These results support the idea that the selection of core transcription factor (e.g. TFIID versus SAGA) may affect the fate of mRNA generated even from a single promoter.

Free Research Field

分子生物学