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2013 Fiscal Year Final Research Report

Mechanisms of cell cycle-dependent conversion of the mother centriole to the basal body

Research Project

  • PDF
Project/Area Number 24657126
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionTohoku University

Principal Investigator

MIZUNO Kensaku  東北大学, 生命科学研究科, 教授 (70128396)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords一次繊毛 / 中心体 / 細胞周期 / 基底小体 / NDR / Rabin8 / Rab8 / 小胞輸送
Research Abstract

Primary cilia are formed by conversion of the mother centriole to the basal body. Ciliary membranes are generated by fusion of vesicles to the pericentrosome, a process requiring Rab11-mediated recruitment of Rabin8, Rab8 activation and Rabin8 binding to Sec15, a component of the exocyst.This study showed that NDR2 phosphorylates Rabin8 at Ser-272 and defects in this phosphorylation impair preciliary membrane assembly and ciliogenesis, resulting in accumulation of Rabin8/Rab11-containing vesicles at the pericentrosome. Rabin8 binds to and colocalizes with GTP-bound Rab11 and phosphatidylserine (PS) on pericentrosomal vesicles. The phospho-mimetic S272E mutation of Rabin8 decreases affinity for PS but increases affinity for Sec15. These results suggest that NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by triggering the switch in binding specificity of Rabin8 from PS to Sec15, thereby promoting local activation of Rab8 and ciliary membrane formation.

  • Research Products

    (16 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Multifaceted roles of Furry proteins in invertebrates and vertebrates2014

    • Author(s)
      Nagai, T., and Mizuno, K.
    • Journal Title

      J. Biochem

      Volume: 155巻 Pages: 137-146

    • Peer Reviewed
  • [Journal Article] Furry promotes acetylation of microtubules in the mitotic spindle by inhibition of SIRT2 tubulin deacetylase2013

    • Author(s)
      Nagai, T., Ikeda, M., Chiba, S., Kanno, S., and Mizuno, K.
    • Journal Title

      J. Cell Sci

      Volume: 126巻 Pages: 4369-4380

    • Peer Reviewed
  • [Journal Article] NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec152013

    • Author(s)
      Chiba, S., Amagai, Y., Homma, Y., Fukuda, M., and Mizuno, K
    • Journal Title

      EMBO J

      Volume: 32巻 Pages: 874-855

    • Peer Reviewed
  • [Journal Article] Cancer susceptibility and embryonic lethality in Mob1A/1B double mutant mice2012

    • Author(s)
      Nishio, M., Hamada, K., Kawahara, K., Sasaki, M., Noguchi, F., Chiba, S., Mizuno, K., Suzuki, S. O., Dong, Y., Tokuda, M., Morikawa, T., Hikasa, H., Eggenschwiler, J., Yabuta, N., Nojima, H., Nakagawa, K., Hata, Y., Nishina, H., Mimori, K., Mori, M., Sasaki, T., Mak, T. W., Nakano, T., Itami, S., and Suzuki, A.
    • Journal Title

      J. Clin. Inv

      Volume: 122巻 Pages: 4505-4518

    • Peer Reviewed
  • [Journal Article] Furry protein promotes Aurora A-mediated Polo-like kinase 1 activation2012

    • Author(s)
      Ikeda, M., Chiba, S., Ohashi, K., and Mizuno, K.
    • Journal Title

      J. Biol. Chem

      Volume: 287巻 Pages: 3707-3721

    • Peer Reviewed
  • [Presentation] NDRによるPI4KIIIßのリン酸化と一次繊毛形成における機能2013

    • Author(s)
      清水祐子、高橋克宣、水野健作
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      20131203-06
  • [Presentation] Furryによるチューブリン脱アセチル化酵素SIRT2の活性制御と分裂期紡錘体微小管のアセチル化に対する役割2013

    • Author(s)
      永井友朗、池田真教、千葉秀平、菅野新一郎、水野健作
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      20131203-06
  • [Presentation] NDR2によるRabin8のリン酸化と一次繊毛形成における機能2013

    • Author(s)
      千葉秀平、天貝佑太、本間悠太、福田光則、水野健作
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      20130911-13
  • [Presentation] NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec152013

    • Author(s)
      Chiba,S., Amagai,Y., Homma,Y., Fukuda,M., Mizuno,K.
    • Organizer
      FASEB Science Research Conferences Biology of Cilia & Flagella
    • Place of Presentation
      Niagara Falls, New York, USA
    • Year and Date
      20130623-28
  • [Presentation] Furry promotes acetylation of microtubules in the mitotic spindle via inhibition of SIRT2 tubulin deacetylase2013

    • Author(s)
      Tomoaki Nagai, Shuhei Chiba, Masanori Ikeda, Shin-ichiro Kanno, Kensaku Mizuno
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      20130619-21
  • [Presentation] NDR2-mediated Rabin8 Phosphorylation is Crucial for Ciliogenesis by Switching Binding Specificity from Phosphatidylserine to Sec152013

    • Author(s)
      Chiba, S., Mizuno, K.
    • Organizer
      第25回CDBミーティング
    • Place of Presentation
      神戸
    • Year and Date
      20130617-18
  • [Presentation] NDR2によるRabin8のリン酸化と一次繊毛形成における機能2013

    • Author(s)
      千葉秀平、天貝佑太、本間悠太、福田光則、水野健作
    • Organizer
      日本生化学会東北支部第79回例会・シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2013-05-11
  • [Presentation] 一次繊毛形成における膜輸送制御 : 繊毛病との関連2013

    • Author(s)
      水野健作、千葉秀平
    • Organizer
      東北大学GCOEシンポジウム「Network Medicineによる医学・生命科学の新たな潮流」
    • Place of Presentation
      仙台
    • Year and Date
      2013-02-01
  • [Presentation] Furry is a positive regulator of microtubule acetylation in mitotic spindle2012

    • Author(s)
      T. Nagai, S. Chiba, M. Ikeda, S-I. Kanno, A.Yasui, K. Ohashi, K. Mizuno
    • Organizer
      第52回American Society for Cell Biology Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      20121213-20
  • [Book] 一次繊毛形成と細胞周期のクロストーク, 実験医学増刊「細胞周期による高次生命現象の制御と疾患」2013

    • Author(s)
      千葉秀平、天貝佑太、水野健作
    • Total Pages
      252-256
    • Publisher
      羊土社
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/mizuno_lab/

URL: 

Published: 2015-06-25  

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