Single molecule imaging and kinetic analysis of actin filament-cofilin interactions modulated by fluctuations of the actin filament.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: HAYAKAWA Kimihide 名古屋大学, 医学(系)研究科(研究院), 特任講師 (60467280)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アクチン / コフィリン / 張力 / ゆらぎ / 協同性

Outline of Final Research Achievements

Actin-depolymerizing factor/cofilin proteins preferentially sever actin filaments when the filament was not tensed. We performed real-time single molecule imaging of the binding of cofilin to single actin filaments without tension. Our results show that the binding of a single cofilin molecule enhances the binding of additional cofilin within 65 nm (across 26 actin protomers) of the initial binding site. The fluctuations of the actin filaments was positively correlated to the on-rate of cofilin binding.

Free Research Field

生物物理