Relationship between lipid droplets and peroxisomes through the mitochondria-associated ER membrane

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657141
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: TAGAYA MITSUO 東京薬科大学, 生命科学部, 教授 (30179569)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 小胞体 / ミトコンドリア / ペルオキシソーム / 脂肪滴 / Syntaxin 17 / Sec16B

Research Abstract

We examined whether the MAM (mitochondria-associated membrane), a subdomain of the endoplasmic reticulum, participates in lipid droplet formation and peroxisome biogenesis. Lipid droplet formation was suppressed by knockdown of MAM-organizing proteins, such as syntaxin17. Rab32 may also contribute to lipid droplet formation. Depletion of Sec16B caused lipid droplet formation and elongation of peroxisomes in a certain cell type, but suppressed lipid droplet formation and shortened peroxisomes in another type of cells. The data suggest that the MAM has an important role in lipid droplet formation, and that there is an intimate relation between lipid droplet formation and peroxisome biogenesis.

Research Products

• [Journal Article] Moonlighting functions of the NRZ (mammalian Dsl1) complex. Front (2014)
  • Author(s): Tagaya, M., Arasaki, K., Inoue, H., and Kimura, H.
  • Journal Title: Cell Develop. Biol Volume: (in press)
  • DOI: 10.3389/fcell.2014.00025
  • Peer Reviewed
• [Journal Article] Phosphatidic acid (PA)- preferring phospholipase A_1 regulates mitochondrial dynamics (2014)
  • Author(s): Baba, T., Kashiwagi, Y., Arimitsu, N., Kogure, T., Edo, A., Maruyama, T., Nakao, K., Nakanishi, H., Kinoshita, M., Frohman, M.A., Yamamoto, A., and Tani, K.
  • Journal Title: J. Biol. Chem Volume: 289 Pages: 11497-11511
  • DOI: 10.1074/jbc.M113.531921
  • Peer Reviewed
• [Journal Article] MAMの新たな機能と関連疾患 (2014)
  • Author(s): 多賀谷光男、若菜裕一、川端美緒
  • Journal Title: 細胞工学(学研メディカル秀潤社) Volume: 33(4) Pages: 436-442
• [Journal Article] Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface (2013)
  • Author(s): Wakana, Y., Villeneuve, J., van Galen, J., Cruz-Garcia, D., Tagaya, M.,and Malhotra, V.
  • Journal Title: J. Cell Biol Volume: 202 Pages: 241-250
  • DOI: 10.1083/jcb.201303163
  • Peer Reviewed
• [Journal Article] オルガネラ接触部位 : 多様な機能と疾患 (2013)
  • Author(s): 多賀谷光男、谷佳津子、新崎恒平
  • Journal Title: 実験医学(羊土社) Volume: 31(11) Pages: 1791-1797
• [Journal Article] Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum (2012)
  • Author(s): Miyazaki, K., Wakana, Y., Noda, C., Arasaki, K., Furuno, A., and Tagaya, M.
  • Journal Title: J. Cell Sci Volume: 125 Pages: 5658-5666
  • DOI: 10.1242/jcs.105304
  • Peer Reviewed
• [Journal Article] Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport (2012)
  • Author(s): Steuble, M., Diep, T. M., Schätzle, P., Ludwig, A., Tagaya, M., Kunz, B., and Sonderegger, P.
  • Journal Title: Biol,Open Volume: 1 Pages: 761-774
  • DOI: 10.1242/bio.20121578
  • Peer Reviewed
• [Journal Article] The intracellular phospholipase A_1 protein family (2012)
  • Author(s): Tani, K., Kogure, T., and Inoue, H.
  • Journal Title: Biomol,Concepts Volume: 3 Pages: 471-478
  • DOI: 10.1515/bmc-2012-0014
  • Peer Reviewed
• [Presentation] 脂肪滴形成および3T3-L1の脂肪細胞への分化におけるsyntaxin 17の関与 (2014)
  • Author(s): 木村葉那、茂刈寛史、新崎恒平、谷佳津子、多賀谷光男
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 20140600
• [Presentation] 小胞の繋留に関与するCATCHRファミリータンパク質間の連携 : RINT-1はCOG複合体と協調してエンドソームからトランスゴルジ・ネットワークへの小胞輸送を調節する (2013)
  • Author(s): 新崎恒平、多賀谷光男
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20131200
• [Presentation] 小胞体とミトコンドリアの接触部位におけるsyntaxin17の役割 (2013)
  • Author(s): 多賀谷光男、新崎恒平
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] Phosphatidic acid-preferring phospholipase A_1(PA-PLA_1)/DDHD1はミトコンドリアの形態形成に関与する (2013)
  • Author(s): 馬場崇、江戸歩実、小笠原裕太、山本章嗣、谷佳津子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] VIMPは微小管結合タンパク質との相互作用を介して小胞体の形態を調整する (2012)
  • Author(s): 野田睦乃、松下充、新崎恒平、多賀谷光男
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] レジオネラエフェクターであるDrrAはER小胞とLCV膜との融合を促進する (2012)
  • Author(s): 新崎恒平、川端美緒、Derek Toomer、多賀谷光男、Craig Roy
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] syntaxin 17はmitochondria-associated membrane(MAM)に局在し、脂肪滴の形成に関与する (2012)
  • Author(s): 清水浩顕、茂刈寛史、西田直貴、新崎恒平、多賀谷光男
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Book] Chapter 5 The Structures and Functions of Intracellular Phospholipase A_1 Family Proteins, in"Phospholipases in Health and Disease", Eds. Tappia, P. S, and Dhalla, N. S. (2014)
  • Author(s): Tani, K., Baba, T., and Inoue, K.
  • Total Pages: 87-99
  • Publisher: Springer Science+Business Media New York