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2012 Fiscal Year Final Research Report

"Visualizing epigenetics" -challenging from Live-cell imaging-

Research Project

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Project/Area Number 24657142
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SAKAUE Asako  独立行政法人理化学研究所, 細胞機能探索技術開発チーム, 研究員 (90462689)

Project Period (FY) 2012
KeywordsDNA methylation / single cell imaging analysis / population analysis / Fucci / cellular heterogeneity
Research Abstract

We developed several genetically encoded probes that monitor DNA methylation states, which we hope will revolutionize our ability to study epigenetic regulation in individual cells. By tuning the affinity of the probe for methylated DNA, we tried to increase the probe response while minimizing the toxicity to recipient cells. Also, we diversified the color of the fluorescent proteins employed in the probe in order to facilitate simultaneous imaging DNA methylation in conjunction with the cell cycle. The microscope system for performing live imaging with a high spatial resolution was built up. We will continue to work on this project in the next Grant-in-Aid for Scientific Research (C) in the H25 fiscal year.

  • Research Products

    (7 results)

All 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.2013

    • Author(s)
      Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.
    • Journal Title

      Development. 2013 Jan 1

      Volume: 1;140(1) Pages: 237-46

    • Peer Reviewed
  • [Journal Article] Nuclear transferred embryonic stem cell for analysis of B1 B-lymphocyte development.2012

    • Author(s)
      Takase M, Iida R, Maruya M, Sakaue-Sawano A, Miyawaki A, Wakayama T, Nishigami S, Fagarasan S, Kanagawa O
    • Journal Title

      Int Immunol. 2012 Oct 6.

    • Peer Reviewed
  • [Journal Article] Mechanism of cancer cell death induced by depletion of an essential replication regulator.2012

    • Author(s)
      Ito S, Ishii A, Kakusho N, Taniyama C, Yamazaki S, Fukatsu R, Sakaue-Sawano A, Miyawaki A, Masai H
    • Journal Title

      PLoS One. 2012

      Volume: 7(5) Pages: e36372

    • Peer Reviewed
  • [Journal Article] ZSTK474, a specific phosphatidylinositol 3-kinase inhibitor, induces G1 arrest of the cell cycle in vivo.2012

    • Author(s)
      Dan S, Okamura M, Mukai Y, Yoshimi H, Inoue Y, Hanyu A, Sakaue-Sawano A, Imamura T, Miyawaki A, Yamori T
    • Journal Title

      Eur J Cancer. 2012 Apr

      Volume: 48(6) Pages: 936-43

    • Peer Reviewed
  • [Presentation] Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression2012

    • Author(s)
      Asako Sakaue-Sawano, Hiroshi Hama & Atsushi Miyawaki
    • Organizer
      国際幹細胞学会 第10回年次大会 ‐ISSCR 2012
    • Place of Presentation
      神奈川県:横浜
    • Year and Date
      20120613-16
  • [Book] 実験医学増刊号(2013) ユビキチンオシレイターによる細胞周期可視化 ー細胞周期を4次元で理解するー2013

    • Author(s)
      阪上―沢野 朝子, 小椋陽介, 笹倉靖徳, 宮脇 敦史
    • Total Pages
      178-185
    • Publisher
      羊土社
  • [Remarks]

    • URL

      http://cfds.brain.riken.jp/Fucci.html

URL: 

Published: 2014-09-25  

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