Imaging the opposite side of lipid raft

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657143
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: KOBAYASHI Toshihide 独立行政法人理化学研究所, 小林脂質生物学研究室, 主任研究員 (60162004)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 脂質ドメイン / 脂質ラフト / 脂質の非対称性 / 脂質プローブ / コレステロール / スフィンゴミエリン / 脂質結合性毒素 / セカンドメッセンジャー

Research Abstract

Lipid raft is a plasma membrane lipid domain enriched with sphingolipids and cholesterol. Although lipid rafts are involved in various physiological phenomena, the detailed structures of lipid rafts are not well elucidated. We have developed novel protein probes that specifically bind lipid rafts. Using these probes and advanced microscope techniques, we showed that the transbilayer movement of lipid second messenger, diacylglycerol, is regulated by sphingomyelin. Our results also indicate the existence of sphingomyelin-rich membrane domains in both outer and inner leaflet of the plasma membranes from cultured fibroblasts.

Research Products

• [Journal Article] Imaging local sphingomyelin-rich domains in the plasma membrane using specific probes and advanced microscopy (2014)
  • Author(s): Abe M, Kobayashi T.
  • Journal Title: Biochim Biophys Acta Volume: 1841 Pages: 720-726
  • DOI: 10.1016/j.bbalip.2013.07.003
  • Peer Reviewed
• [Journal Article] Lipid compartmentalization in the endosome system (2014)
  • Author(s): Hullin-Matsuda F, Taguchi T, Greimel P, Kobayashi T.
  • Journal Title: Sem Cell Dev Biol Volume: (in press)
  • DOI: 10.1016/j.semcdb.2014.04.010
  • Peer Reviewed
• [Journal Article] Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides (2013)
  • Author(s): Nishimura S, Ishii K, Iwamoto K, Arita Y, Matsunaga S, Ohno-Iwashita Y, Sato SB, Kakeya H, Kobayashi T, Yoshida M.
  • Journal Title: PLoS ONE Pages: 8-e83716
  • DOI: 10.1371/journal.pone.0083716
  • Peer Reviewed
• [Journal Article] Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains (2013)
  • Author(s): Bhat HB, Kishimoto T, Abe M, Makino A, Inaba T, Murate M, Dohmae N, Kurahashi A, Nishibori K, Fujimori F, Greimel P, Ishitsuka R, Kobayashi T.
  • Journal Title: J Lipid Res Volume: 54 Pages: 2933-2943
  • DOI: 10.1016/j.bpj.2013.07.052
  • Peer Reviewed
• [Journal Article] Real time visualization of assembling of a sphingomyelin-specific toxin on planar lipid membranes (2013)
  • Author(s): Yilmaz N, Yamada T, Greimel P, Uchihashi T, Ando T, Kobayashi T.
  • Journal Title: Biophys J Volume: 105 Pages: 1397-1405
  • DOI: 10.1016/j.bpj.2013.07.052
  • Peer Reviewed
• [Journal Article] Sphingomyelin regulates the transbilayer movement of diacylglycerol in the plasma membrane of Madin-Darby canine kidney cells (2013)
  • Author(s): Ueda Y, Makino A, Murase-Tamada K, Sakai S, Inaba T, Hullin-Matsuda F, Kobayashi T.
  • Journal Title: FASEB J Volume: 27 Pages: 3284-3297
  • DOI: 10.1096/fj.12-226548
  • Peer Reviewed
• [Journal Article] Subcellular localization of sphingomyelin revealed by two toxin-based probes in mammalian cells (2012)
  • Author(s): Yachi R, Uchida Y, Balakrishna, BH, Anderluh G, Kobayashi T, Taguchi T, Arai H.
  • Journal Title: Genes Cells Volume: 17 Pages: 720-727
  • DOI: 10.1111/j.1365-2443.2012.01621
  • Peer Reviewed
• [Presentation] 脂質のイメージング (2014)
  • Author(s): 小林俊秀
  • Organizer: 第1回JFAS講演会
  • Place of Presentation: 秋葉原
  • Year and Date: 2014-01-11
• [Presentation] 「膜を横切る脂質の運動、フリップフロップについて」Workshop : CROSSroads of Users and J-PARC (2013)
  • Author(s): 小林俊秀
  • Organizer: 第10回「生体膜・コロイド研究の最前線」
  • Place of Presentation: 茨城
  • Year and Date: 20131218-19
• [Presentation] Free cholesterol loading induces the degradation of perilipin 2 and rab18-de pendent abnormal lipidation of apolipoprotein B in cultured hepatocytes (2013)
  • Author(s): Makino A, Hullin-Matsuda F, Murate M, Abe M, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton-Vandenbroucke I, Kobayashi T.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20130911-13
• [Presentation] 細胞分裂時におけるホスファチジルイノシトール4,5_二リン酸の分裂溝への局在に対するスフィンゴミエリンドメインの役割 (2013)
  • Author(s): 小林俊秀、阿部充宏、牧野麻美、ウラン松田フランソワーズ
  • Organizer: 第90回生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130327-29
• [Presentation] "Imaging lipids and lipid domains"2^<nd> Mini Symposium"Protein-lipid interactions" (2013)
  • Author(s): Kobayashi T.
  • Organizer: Piran
  • Place of Presentation: Slovenia
  • Year and Date: 2013-09-06
• [Presentation] 細胞質分裂におけるスフィンゴミエリンドメインの役割 (2012)
  • Author(s): 阿部充宏、牧野麻美、ウラン松田フランソワーズ、小林俊秀
  • Organizer: 第85回生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-16
• [Presentation] エンドソーム特異的脂質の構造、代謝、機能 (2012)
  • Author(s): 小林俊秀
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 福岡
  • Year and Date: 20120607-08
• [Remarks]
  • URL: http://www.riken.jp/lbl/mainpagelbl.html