2013 Fiscal Year Final Research Report
Imaging the opposite side of lipid raft
| Project/Area Number |
24657143
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KOBAYASHI Toshihide 独立行政法人理化学研究所, 小林脂質生物学研究室, 主任研究員 (60162004)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脂質ドメイン / 脂質ラフト / 脂質の非対称性 / 脂質プローブ / コレステロール / スフィンゴミエリン / 脂質結合性毒素 / セカンドメッセンジャー |
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| Research Abstract |
Lipid raft is a plasma membrane lipid domain enriched with sphingolipids and cholesterol. Although lipid rafts are involved in various physiological phenomena, the detailed structures of lipid rafts are not well elucidated. We have developed novel protein probes that specifically bind lipid rafts. Using these probes and advanced microscope techniques, we showed that the transbilayer movement of lipid second messenger, diacylglycerol, is regulated by sphingomyelin. Our results also indicate the existence of sphingomyelin-rich membrane domains in both outer and inner leaflet of the plasma membranes from cultured fibroblasts.
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[Journal Article] Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains2013
Author(s)
Bhat HB, Kishimoto T, Abe M, Makino A, Inaba T, Murate M, Dohmae N, Kurahashi A, Nishibori K, Fujimori F, Greimel P, Ishitsuka R, Kobayashi T.
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Journal Title
J Lipid Res
Volume: 54
Pages: 2933-2943
DOI
Peer Reviewed
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[Presentation] Free cholesterol loading induces the degradation of perilipin 2 and rab18-de pendent abnormal lipidation of apolipoprotein B in cultured hepatocytes2013
Author(s)
Makino A, Hullin-Matsuda F, Murate M, Abe M, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton-Vandenbroucke I, Kobayashi T.
Organizer
第86回日本生化学会大会
Place of Presentation
横浜
Year and Date
20130911-13
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