2013 Fiscal Year Final Research Report
Development of High sensitive and high-resolution detection of extra-celluar ATP in mouse and searching ATP signal in mouse embryo
| Project/Area Number |
24657144
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Gunma University |
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Principal Investigator |
YAMAMOTO Masamichi 群馬大学, 先端科学研究指導者育成ユニット, 助教 (70423150)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アデノシン三リン酸 / 細胞外 |
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| Research Abstract |
To detect the low density and extra-celluar ATP, ATeamL was developed. ATeamL was constructed in FRET-based ATP indicator by genetically linking GFP and Kusabira Orange with epsilon subunit , which was derived from Bacillus (high affinity to ATP). ATeamL, connected to membrane anchoring sequence, was displayed on extra-celluar region in HeLa cell. Now, I generate the transgenic mouse using this construct to be able to detect the extra-celluar ATP in vivo.
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