2013 Fiscal Year Final Research Report
Towards the creation of transgenic snails for the identification of the handedness determining gene
| Project/Area Number |
24657149
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
KURODA Reiko 東京理科大学, 総合研究機構, 教授 (90186552)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 発生遺伝 |
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| Research Abstract |
In order to develop methods to produce transgenic snails, the techniques of gene transfer into early embryos and of generating expression vectors which can express a foreign gene in germ cells were investigated. With the use of an expression vector containing the endogenous beta-actin promoter sequence, we have succeeded in increasing the survival rate of embryos containing injected exogenous DNA. I-SceI meganuclease method was also applied to obtain the transgenic snails.
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