2012 Fiscal Year Final Research Report
Induction of hematopoietic stem cells from ES/iPS cellswithout gene manipulation
| Project/Area Number |
24657157
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
OGAWA Minetaro 熊本大学, 発生医学研究所, 教授 (70194454)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAMOTO Hiroshi 熊本大学, 発生医学研究所, 助教 (00347014)
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| Project Period (FY) |
2012
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| Keywords | 幹細胞 |
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| Research Abstract |
This study aimed at establishing a defined culture condition whichallows ES cells to differentiate into long-term reconstituting hematopoietic stem cellswithout gene manipulation. Multi-potent hematopoietic progenitor cells were able to beinduced from ES cells in a serum-free culture in the presence of several growth factors andchemicals. When transplanted into lethally irradiated adult mice, however , thesehematopoietic progenitor cells failed to show an ability of long-term reconstitution of bonemarrow hematopoiesis.
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