2013 Fiscal Year Final Research Report
Development of a high-efficient gene targeting technique
| Project/Area Number |
24658005
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
MURATA MINORU 岡山大学, その他部局等, 教授 (20166292)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術専門職員 (60379807)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 遺伝子ターゲッティング / 染色体工学 / Cre/LoxP |
|---|
| Research Abstract |
To aim at establishing a high-efficient homologous recombination system in higher plants, we investigated a relationship between occurrence of circularized T-DNA and transformation efficiency. A co-infection with a target construct and a Cre-expressing construct to Arabidopsis seedlings was found to be effective in the circularization of T-DNA and integration into chromosomes.
|
