Production of stress-tolerant wheat by chloroplast transformation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658010
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: Kyoto Sangyo University
• Principal Investigator: TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192)
• Co-Investigator(Kenkyū-buntansha): YAMAGISHI Hiroshi 京都産業大学, 総合生命科学部, 教授 (10210345)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: wheat / chloroplast / transformation / apx / stress-tolerance

Outline of Final Research Achievements

Chloroplast transformation technology has been developed for Solanaceae crops such as tobacco. In order to obtain the first transplastomic wheat, we applied this technology to wheat. We tried to produce the transplastomic wheat that is tolerant to abiotic stresses such as strong light and drought. Since the plant often produces harmful reactive oxygen species under abiotic stresses, we wanted to make an over expresser of chloroplast ascorbate peroxidase. Apx gene encoding ascorbate peroxidase was cloned into wheat chloroplast transformation vectors, and vector DNAs were delivered to the immature embryo-derived callus by the particle bombardment system, then recombinants were selected on the plate containing antibiotics. Although about 54,000 immature embryos were prepared and used in the experiment, no transformant was obtained.

Free Research Field

plant organelle genetics