Reproduction barrier in interploidy crosses in Vitis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658025
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: TAO Ryutaro 京都大学, (連合)農学研究科(研究院), 准教授 (10211997)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 果樹 / ブドウ / 倍数性 / ゲノムインプリンティング / 次世代シークエンシング技術 / 受粉受精 / 胚発生 / 胚乳発生

Research Abstract

Seed formation after inter- and intra- ploidy crosses were investigated in Vitis. Crosses were made between a European grape cultivar Rosaki (V. vinifera), a rootstock cultivar 5BB (V. riparia x V. berlandieri), and chromosome doubled clones produced from Rosaki and 5BB by colochicine treatment. Differences in endosperm cells were observed among crosses 30 days after flowering. Accumulation of protein bodies in endosperm at this stage correlated with the seed germination ability after seed maturation. RNA-Seq analysis was performed with developing seeds. Stress related gene expressions were high in inter-ploidy crosses.