Development of an evaluation system for inducibility of strawberry allergy.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658030
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kobe University
• Principal Investigator: UNO Yuichi 神戸大学, (連合)農学研究科(研究院), 准教授 (90304120)
• Co-Investigator(Kenkyū-buntansha): NABE Takeshi 京都薬科大学, 薬学部, 准教授 (40228078) ; NITTA Yoko 岡山県立大学, 保健福祉学部, 准教授 (70403318) ; TSURUTA Hiroki 神戸大学, 連携創造本部, 准教授 (20346282)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: イチゴ / バラ科果実 / 品種間差 / 栽培方法 / シラカバ花粉症 / 口腔アレルギー症候群

Research Abstract

Fruit allergies are serious health problems that limit the quality of life and slumping consumption of agricultural products. Strawberry can cause oral allergy syndrome, an allergic reaction in patients. In this study, the fundamental knowledge was obtained by analyzing strawberry allergen Fra a and antiallergic constituents, towards the development of an evaluation system for inducibility of strawberry allergy. The analysis of IgE binding capacity revealed that Fra a 1 might plays an important role in determining the allergenicity of strawberry fruits. Fra a 1 content varied by cultivars, cultivating methods, ripening stages, and different tissues. The secondary structure of Fra a 1 was altered by heating to more than 60 C. There is a difference in antiallergic effects among strawberry cultivars. These findings suggest that strawberry allergy symptoms could be reduced by appropriate selection of cultivars, cultivating methods, and cooking methods.

Research Products

• [Journal Article] Expression of strawberry allergen Fra a 1 gene during fruit ripening (2014)
  • Author(s): Futsuki, D., Nitta, Y., Iduhara, M., Tsuruta, H., Tsugehara, T. and Uno, Y.
  • Journal Title: Acta Horticulturae Volume: (in press)
  • Peer Reviewed
• [Journal Article] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1 (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Journal Title: International Strawberry Congress, Antwerp Volume: (USB) Pages: 6
• [Journal Article] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Journal Title: International Strawberry Congress, Antwerp Volume: (USB) Pages: 6
• [Journal Article] The temperature dependence of Fra a 1 protein structure (2012)
  • Author(s): Nitta, Y., Futsuki, D., Nabe, T., Tsuruta, H., Iduhara, M., Uno, Y. and Matsuo, K.
  • Journal Title: HiSOR Activity Report 2012 Pages: 132-133
• [Presentation] イチゴアレルゲンFra a 1のIgE反応性と果実における局在 (2013)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,厳原美穂,野口裕司,宇野雄一
  • Organizer: 園芸学会平成25年度秋季大会
  • Place of Presentation: 岩手大学,園芸学研究
  • Year and Date: 20130920-22
• [Presentation] Analysis of IgE binding capacity and stress inducibility of strawberry allergen Fra a 1, Book of abstracts (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Organizer: International Strawberry Congress
  • Place of Presentation: Antwerp (Belgium)
  • Year and Date: 20130904-06
• [Presentation] Comparison of transcript and protein levels of strawberry allergen Fra a 1 among different cultivars,V (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: International Strawberry Congress
  • Place of Presentation: Antwerp (Belgium)
  • Year and Date: 20130904-06
• [Presentation] Comparison of IgE binding capacity and expression analysis of strawberry allergen Fra a 1 (2013)
  • Author(s): Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M. and Uno, Y.
  • Organizer: ASHS (American Society for Horticultural Science) Annual Congress
  • Place of Presentation: Palm Desert (California, USA)
  • Year and Date: 20130722-25
• [Presentation] Varietal differences in transcript and protein levels of strawberry allergen Fra a 1 (2013)
  • Author(s): Narukami, M., Futsuki, D., Nabe, T., Nitta, Y., Tsuruta, H., Yamazaki, K., Iduhara, M., Noguchi, Y. and Uno, Y.
  • Organizer: ASHS (American Society for Horticultural Science) Annual Congress
  • Place of Presentation: Palm Desert (California, USA)
  • Year and Date: 20130722-25
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の単離と発現解析 (2012)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,厳原美穂,宇野雄一
  • Organizer: 園芸学会平成24年度秋季大会
  • Place of Presentation: 福井県立大学,園芸学研究
  • Year and Date: 20120922-24
• [Presentation] イチゴアレルゲンをコードするFra a 1遺伝子の解析 (2012)
  • Author(s): 夫津木大輔,奈邉健,新田陽子,鶴田宏樹,山崎清志,厳原美穂,宇野雄一
  • Organizer: 神戸大学研究基盤センター若手フロンティア研究会
  • Place of Presentation: 神戸大学
  • Year and Date: 2012-12-25