2013 Fiscal Year Final Research Report
Can transgenic rootstock accelerated flowering transduce flowering signal to scion?
| Project/Area Number |
24658034
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
WADA Masato 独立行政法人農業・食品産業技術総合研究機構, 果樹研究所リンゴ研究領域, 上席研究員 (40241773)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 花成 / リンゴ / 台木 / 接ぎ木 |
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| Research Abstract |
In the signal transduction experiments of flowering on apple, transgenic apples by rolC:AtFT were rootstocks, and nontransgenic apples were scions. They were grafted with changing length of scions. For 2 years of the experiment, no flower induction of scions were observed in spite of lengths. Bud grafting has been tried, we did not have the results in this research period. MdTFL1 suppressed rootstocks by silencing were used for same grafting experiment to detect silencing signal transduction. Nontransgenic scions have not promoted flowering. In other experiment, two shoots from nontransgenic apple was grafted rolC:AtFT transgenic apple to one shoot and other shoot were removed all leaves. The shoot without leaves seemed to increase sink ability, but the shoot did not set flowers.
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[Journal Article] Effects of microspore development stage and cold pre-treatment of flower buds on embryo induction in apple (Malus × domestica Borkh.) anther culture2013
Author(s)
Chunfen Zhang, Tasuro Tsukuni, Miyuki Ikeda, Mamoru Satoh, Hatsuhiko Okada, Yoshitaka Ohashi, Hideyuki Matsuno, Toshiya Yamamoto, Masato Wada, Nobuyuki Yoshikawa, Shogo Matsumoto, Jijun Li, Naozumi Mimida, Manabu Watanabe, Akira Suzuki, Sadao Komori
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Journal Title
J. Japan. Soc. Hort. Sci.
Volume: 82
Pages: 114-124
DOI
Peer Reviewed
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[Journal Article] Application of an Analog of 9, 10-ketol-octadecadienoic acid (KODA), Affected Flower Bud Formation and MdTFL1 and MdFT1 Gene Expressions in Apple Buds under Heavy-crop and Shade Conditions2013
Author(s)
Monrudee Kittikorn, Katsuya Okawa, Hitoshi Ohara, Satoru Kondo, Nobuhiro Kotoda, Masato Wada, Mineyuki Yokoyama, Ohji Ifuku, Ariake Murata and Naoharu Watanabe
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Journal Title
J. Japan. Soc. Hort. Sci.
Volume: 138(2)
Pages: 102-107
URL
Peer Reviewed
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[Journal Article] Divergence of TERMINAL FLOWER1-like genes in Rosaceae2012
Author(s)
Naozumi Mimida, Jijun Li, Chunfen Zhang, Shigeki Moriya, Yuki Moriya-Tanaka, Hiroshi Iwanami, Chikako Honda, Hidemi Oshino, Kaori Takagishi, Akira Suzuki, Sadao Komori, Masato Wada
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Journal Title
Biologia Plantarum
Volume: 56(3)
Pages: 465-472
URL
Peer Reviewed
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