2014 Fiscal Year Final Research Report
Development of Neurospora crassa as a model filamentous fungus for studying virus/host interactions.
| Project/Area Number |
24658041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | マイコウイルス / トランスフェクション / 宿主範囲 / 逆遺伝学 / dsRNA / RNAウイルス / RNAサイレンシング |
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| Outline of Final Research Achievements |
As a model system for genetics, biochemistry or developmental biology, Neurospora crassa has greater advantages over any other filamentous fungi due to its even short life cycle time, ease of crossing, and well-established tools and resources represented by a number of gene knockout strains available from the Fungal Genetics Stock Center. This study aimed at screening of previously reported fungal viruses for N. crassa-infectious viruses. We confirmed that N. crassa supports the replication of two heterologous viruses, mycoreovirus 1 (MyRV1) and Rosellinia necatrix partitivirus 2 (RnPV2) from other ascomycetes, Cryphonectrial parasitica and R. necatrix. The two viruses could be maintained not only in the wild-type strain but also RNA silencing defective mutants of N. crassa. It is now being investigated what effects of deletions of RNA silencing-related genes on virus replication are, and how these genes respond to virus infection.
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| Free Research Field |
ウイルス学
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