Induction of Hypersensitive Response and Susceptibility by Protease Effector from Ralstonia solanacearum

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658042
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: ICHINOSE Yuki 岡山大学, その他の研究科, 教授 (50213004)
• Co-Investigator(Kenkyū-buntansha): MUKAIHARA Takafumi 岡山県農林水産総合センター, 生物研, 専門研究員 (80344406)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 非病原力因子 / エフェクター / 過敏感反応 / 病原性 / Zn依存性プロテアーゼ / 非宿主

Research Abstract

Ralstonia solanacearum causes bacterial wilt disease in more than 200 plant species, including economically important Solanaceae species. R. solanacearum injects virulence factor proteins effectors into plant cytoplasm, then induces hypersensitive response (HR) in nonhost plants and disease in host plants. In this study we identified one effector Rip36 induces HR in the nonhost wild eggplant Solanum torvum in an Hrp-dependent manner. Rip36-induced HR requires the putative Zn-protease motif. On the other hand, Rip36 is a dispensable virulence factor to cause disease in eggplant, because rip36-defective mutants grew well in host eggplants as the wild type.

Research Products

• [Journal Article] Ralstonia solanacearum type III secretion system effector Rip36 induces hypersensitive response in the nonhost wild eggplant Solanum torvum (2014)
  • Author(s): Nahar, K., Matsumoto, I., Taguchi, F., Inagaki, Y., Yamamoto, M., Toyoda, K., Shiraishi, T., Ichinose, Y. and Mukaihara, T.
  • Journal Title: Mol Plant Pathol Volume: 15 (3) Pages: 297-303
  • DOI: 10.1111/mpp.12079
• [Presentation] Ralstonia solanacearum effector Rip36 induces strong immune response in the nonhost wild eggplant (2014)
  • Author(s): Yuki ICHINOSE, Fumiko TAGUCHI
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 20140326-28
• [Presentation] HR-inducing activity of HopH1 from Pseudomonas syringae (2013)
  • Author(s): Kamrun Nahar, Fumiko Taguchi, Yoshishige Inagaki, Mikihiro Yamamoto, Yuki Ichinose, Takafumi Mukaihara
  • Organizer: 平成25年日本植物病理学会関西部会
  • Place of Presentation: 岡山
  • Year and Date: 20130926-27
• [Presentation] 青枯病菌Ralstonia solanacearumにおける宿主域変異機構の解析 (2013)
  • Author(s): 向原隆文、小田賢司
  • Organizer: 日本植物病理学会平成25年度大会
  • Place of Presentation: 岐阜
  • Year and Date: 20130327-29
• [Presentation] Analysis of hypersensitive response of Solanum torvum Sw. cv. Torubamubiga induced by Ralstonia solanacearum Rip36 and Pseudomonas syringae HopH1 effectors (2013)
  • Author(s): Kamrun Nahar, Iyo Matsumoto, Eriko Suetsugu, Fumiko Taguchi, Yuki Ichinose, Takafumi Mukaihara
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] HopH1, a putative Zn-dependent protease, of Pseudomonas syringae induces hypersensitive response in non-host Solanum torvum Sw. cv. Torubamubiga (2012)
  • Author(s): Kamrun Nahar, Iyo Matsumoto, Fumiko Taguchi, Yoshishige Inagaki, Takafumi Mukaihara, Yuki Ichinose
  • Organizer: Symposium on Interdisciplinary Researches in Okayama
  • Place of Presentation: 岡山
  • Year and Date: 20121120-21