2013 Fiscal Year Final Research Report
Mapping of mutation point on holokinetic chromosome in Bombyx mori
| Project/Area Number |
24658046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | Iwate University |
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Principal Investigator |
SAHARA Ken 岩手大学, 農学部, 教授 (30241368)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | BAC-FISH / 染色体突然変異 / 相互転座 / 付着 / カイコゲノム |
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| Research Abstract |
I have analyzed chromosome mutation in 3 Bombyx mori strains (r501, r52 and r53) provided through national bioresource project (NBRP). BAC-FISH (fluorescence in situ hybridization using bacterial artificial chromosomes) revealed the exact direction of the fusion between chromosomes 23 and 25. I defined reciprocal translocation is the cause for the mutation in r52 and r53 strains. The double strand breakage and further fusion for each mutation chromosomes were also analyzed by the BAC-FISH. These detected a bridge BAC responsible for a mutation chromosome. I am trying to reveal the mutation point by PCR as a reference of Bombyx mori genome databes.
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