2014 Fiscal Year Final Research Report
Characterization of an N3 compound-degrading enzyme
| Project/Area Number |
24658070
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMANO Takuto 筑波大学, 生命環境系, 助教 (70585025)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 微生物 / 酵素 / 代謝 / 分解 |
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| Outline of Final Research Achievements |
The N3 compounds composed of three nitrogen atoms joined together by double bound (R-N3), are known to be extremely toxic and explosive. However, the metabolic pathway of N3 compounds has been unknown. We isolated a microorganism that degrades an N3 compound from soil and discovered a novel enzyme involved in the N3 compound metabolism. Also, we examined the culture conditions to increase enzyme production in the cells, and found the optimum culture condition.
Because the enzyme was very unstable, we carried out the purification of the enzyme many times. As a result, the enzyme was purified to homogeneity, through several steps from a cell-free extract of the strain cultured under the optimum culture condition. Some properties of this enzyme were investigated, and it’s subunit structure, optimum temperature and thermal stability were determined.
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| Free Research Field |
応用微生物学
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