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2013 Fiscal Year Final Research Report

Microbial ecology of high-temperature compost and its usefulness as genetic resource

Research Project

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Project/Area Number 24658075
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionMie University

Principal Investigator

SAKKA Kazuo  三重大学, 生物資源学研究科, 教授 (20154031)

Co-Investigator(Kenkyū-buntansha) SAKKA Makiko  三重大学, 大学院生物資源学研究科, 学術研究員 (00422882)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords堆肥 / 好熱性細菌 / 好熱性酵素 / 微生物生態学 / 菌叢 / 細胞壁分解酵素 / 遺伝子資源
Research Abstract

Temperature reached over 90 degrees centigrade in the upper position of the compost of the high-temperature composting system. Viable cell numbers by surface plate method was about 1/100 to 1/1000 of those by microscopic observation method, suggesting that unculturable bacteria were important in the high-temperature composting process. Bacillus species dominated in the early stage of composting, accounting for 36% of the total cell numbers, but Melghirimyces thermohalophilus accounted for 63%, indicating that bacterial flora drastically changed in the process of the composting. Genes encoding thermophilic enzymes were directly cloned from DNA isolated from the compost and thermophilic bacteria producing bacterial cell wall-degrading enzymes were isolated from the compost, indicating that the high-temperature compost was quite useful as the genetic resource for isolating thermophilic enzymes and bacteria.

  • Research Products

    (2 results)

All 2014 2013

All Presentation (2 results)

  • [Presentation] 高温性堆肥の経時的・空間的温度変化に伴う菌叢変化2014

    • Author(s)
      粟冠真紀子、福丸琢人、東俊之、千葉悠佳、木村哲哉、粟冠和郎
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(川崎市多摩区)
    • Year and Date
      2014-03-29
  • [Presentation] 超高温性堆肥の温度と微生物群の経時的・空間的変化の解析2013

    • Author(s)
      福丸琢人、粟冠真紀子、坂井勝、木村哲哉、粟冠和郎
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市中区)
    • Year and Date
      2013-09-20

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Published: 2015-06-25  

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