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2013 Fiscal Year Final Research Report

Bioresource serch from extreme environment with virome mining

Research Project

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Project/Area Number 24658083
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyushu University

Principal Investigator

DOI Katsumi  九州大学, (連合)農学研究科(研究院), 講師 (40253520)

Co-Investigator(Kenkyū-buntansha) TASHIRO Kosuke  九州大学, 大学院農学研究院, 准教授 (00192170)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsphage / Thermus / Inoviridae / replication / genome
Research Abstract

Isolation of thermophilic bacteriophages were carried out with geothermal waters and sediments in hot springs from Obama hot spring and Komatsu-jigoku hot spring. Isolated phages, phi OH3, phi OH16, phi KJ3, phi KJ4 and phi KJ5, were examined with plaque formation, morphology, host range, replication and genome structure. All isolated phages were categorized into Inoviridae, and predicted ORFs on these phages showed high similarities together. These results suggested Thermus filamentous phages are resemble together, regardless of isolating points.

  • Research Products

    (34 results)

All 2014 2013 2012 Other

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (11 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Biochemical characterization of an L-tryptophan dehydrogenase from the photoautotrophic cyanobacterium Nostoc punctiforme2014

    • Author(s)
      R. Ogura, T. Wakamatsu, Y. Mutaguchi, K. Doi and T. Ohshima
    • Journal Title

      Enzyme Microb. Tech.

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Wild mushroom in Nepal : Some potential candidates as antioxidant and ACE inhibition sources2014

    • Author(s)
      T. H. Bang, H. Suhara, K. Doi, H. Ishikawa, K. Fukami, G. P. Parajuli, Y. Katakura, S. Yamashita, K. Watanabe, M. K. Adhikari, H. K. Manandhar, R. Kondo and K. Shimizu
    • Journal Title

      Evid. Based Complement. Alternat. Med.

      Volume: Article ID 195305

    • Peer Reviewed
  • [Journal Article] Efficient synthesis of D-branched-chain amino acids and their labeled compounds with stable isotopes using D-amino acid dehydrogenase2014

    • Author(s)
      H. Akita, H. Suzuki, K. Doi and T. Ohshima
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: Vol. 98, No. 3 Pages: 1135-1143

    • Peer Reviewed
  • [Journal Article] A ternary conjugation system to construct DNA libraries in Geobacillus kaustophilus HTA426, Biosci2013

    • Author(s)
      H. Suzuki, K. Wada, M. Furukawa, K. Doi and T Ohshima
    • Journal Title

      Biotechnol. Biochem.

      Volume: Vol. 77, No.11 Pages: 2316-2318

    • Peer Reviewed
  • [Journal Article] Draft Genome Sequence of Geobacillus kaustophilus GBlys, a lysogenic strain with bacteriophageφOH22013

    • Author(s)
      K. Doi, K. Mori, H. Martono, Y. Nagayoshi, Y. Fujino, K. Tashiro, S. Kuhara and T. Ohshima
    • Journal Title

      Genome A.

      Volume: Vol. 1 Pages: e00634-13

    • Peer Reviewed
  • [Journal Article] Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis2013

    • Author(s)
      T. Wakamatsu, C. Higashi, T. Ohmori, K. Doi and T. Ohshima
    • Journal Title

      Extremophiles

      Volume: Vol. 17, No. 3 Pages: 379-389

    • Peer Reviewed
  • [Journal Article] Distribution of D-amino acids in vinegars and involvement of lactic acid bacteria in the production of D-amino acids2013

    • Author(s)
      Y. Mutaguchi, T. Ohmori, H. Akano, K. Doi and T. Ohshima
    • Journal Title

      SpringerPlus

      Volume: Vol.2 Pages: 691

    • Peer Reviewed
  • [Journal Article] Novel amino acid racemase, isoleucine 2-epimerase, from Lactobacillus species : Identification, purification and characterization2013

    • Author(s)
      Y. Mutaguchi, T. Ohmori, T. Wakamatsu, K. Doi, and T. Ohshima
    • Journal Title

      J. Bacteriol.

      Volume: Vol. 195, No. 22 Pages: 5207-5215

    • Peer Reviewed
  • [Journal Article] Identification of thermo tolerant lactic acid bacteria isolated from silage prepared in the hot and humid climate of Southwestern Japan2013

    • Author(s)
      K. Doi, Y. Nishizaki, H. Kimura, M. Kitahara, Y. Fujino, S. Ohmomo, T. Ohshima and S. Ogata
    • Journal Title

      SpringerPlus

      Volume: Vol. 2 Pages: 485

    • Peer Reviewed
  • [Journal Article] Draft Genome Sequence of D-branched-chain amino acids producing Lactobacillus otakiensis JCM 15040^T isolated from a traditional Japanese pickle2013

    • Author(s)
      K. Doi, K. Mori, Y. Mutaguchi, K. Tashiro, Y. Fujino, T. Ohmori, S. Kuhara and T. Ohshima
    • Journal Title

      Genome A.

      Volume: Vol. 1 Pages: e00546-13

    • Peer Reviewed
  • [Journal Article] Identification and characterization of lactic acid bacteria isolated from fermented rice bran product2013

    • Author(s)
      K. Doi, O. T. A. Phuong, F. Kawatou, Y. Nagayoshi, Y. Fujino and T. Ohshima
    • Journal Title

      Adv. Microbiol.

      Volume: Vol. 3 Pages: 265-272

    • Peer Reviewed
  • [Journal Article] Characterization of D-amino acid aminotransferase from Lactobacillus salivarius2013

    • Author(s)
      J. Kobayashi, Y.Shimizu, Y. Mutaguchi, K. Doi and T. Ohshima
    • Journal Title

      J. Mol. Catal. B : Enzym

      Volume: Vol. 94 Pages: 15-22

    • Peer Reviewed
  • [Journal Article] Draft Genome Sequence of Pediococcus lolii NGRI 0510Q^T isolated from ryegrass silage2013

    • Author(s)
      K. Doi, K. Mori, K. Tashiro, Y. Fujino, Y. Nagayoshi, Y. Hayashi, S. Kuhara and T Ohshima
    • Journal Title

      Genome A.

      Volume: Vol.1 Pages: 00156-12

    • Peer Reviewed
  • [Journal Article] Expression analysis of the spi gene in the pock-forming plasmid pSA1.1 from Streptomyces azureus and localization of its product during differentiation2012

    • Author(s)
      K. Doi, Y. Ohyama, E. Yokoyama, T. Nishiyama, Y. Fujino, Y. Nagayoshi, T. Ohshima and S. Ogata
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: Vol.95, No. 3 Pages: 707-716

    • Peer Reviewed
  • [Journal Article] Silica deposition induced by isolated aluminum ions bound on chelate resin as a model compound of the surface of microbes2012

    • Author(s)
      S. Bai, G. Naren, H. Noma, M. Etou, H. Ohashi, Y. Fujino, K. Doi, Y. Okaue, T. Yokoyama
    • Journal Title

      Colloid Surfaces B

      Volume: Vol.95 Pages: 208-213

    • Peer Reviewed
  • [Journal Article] Effect of microorganisms on flocculation of quartz2012

    • Author(s)
      T. Hirajima, Y. Aiba, M.Farahat, N. Okibe, K. Sasaki, T. Tsuruta and K. Doi
    • Journal Title

      Int. J. Miner. Process.

      Volume: Vol.102-103 Pages: 107-111

    • Peer Reviewed
  • [Journal Article] A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila : Purification, characterization, gene sequencing and crystal structure analysis2012

    • Author(s)
      Y. Zhao, T. Wakamatsua, K. Doi, H. Sakuraba and T. Ohshima
    • Journal Title

      J. Mol. Catal. B : Enzym

      Volume: Vol.83 Pages: 65-72

    • Peer Reviewed
  • [Journal Article] Effects of alkali or acid treatment on the isomerization of amino acids2012

    • Author(s)
      T. Ohmori, Y. Mutaguchi, K. Doi and T. Ohshima
    • Journal Title

      J. Biosci. Bioeng.

      Volume: Vol.114, No. 4 Pages: 457-459

    • Peer Reviewed
  • [Journal Article] Creation of a thermostable NADP(+)-dependent D : -amino acid dehydrogenase from Ureibacillus thermosphaericus strain A1 meso-diaminopimelate dehydrogenase by site-directed mutagenesis2012

    • Author(s)
      H. Akita, K. Doi, Y. Kawarabayasi and T. Ohshima
    • Journal Title

      Biotechnol. Lett.

      Volume: Vol.34, No. 9 Pages: 1693-1699

    • Peer Reviewed
  • [Journal Article] 環境応答とバイオミネラリゼーション-極限環境下における細菌の生存戦略

    • Author(s)
      藤野泰寛、横山拓史、土居克実
    • Journal Title

      生物と化学

      Volume: 50巻 Pages: 175-181

  • [Presentation] 小浜温泉より単離したThermus属繊維状ファージの特性解析2013

    • Author(s)
      相川浩輝、永吉佑子、藤野泰寛、大島敏久、土居克実
    • Organizer
      日本生物工学会九州支部大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2013-12-07
  • [Presentation] 発酵食品に含まれるアミノ酸の研究2013

    • Author(s)
      大森勇門、牟田口祐太、守﨑香菜、土居克実、大島敏久
    • Organizer
      農芸化学会中四国支部第16回若手シンポジウム
    • Place of Presentation
      高知大学
    • Year and Date
      2013-11-02
  • [Presentation] 超好熱菌の2つのNAD(P)-グルタミン酸脱水素酵素の機能開発2013

    • Author(s)
      若松泰介、芦内誠、土居克実、大島敏久
    • Organizer
      ビタミンB研究協議会
    • Place of Presentation
      名古屋・ウインクあいち
    • Year and Date
      2013-11-02
  • [Presentation] 乳酸菌Lactobacillus属由来新規酵素、イソロイシン2-エピメラーゼ2013

    • Author(s)
      牟田口祐太、大森勇門、若松泰介、土居克実、大島敏久
    • Organizer
      日本生物高分子学会2013年度大会
    • Place of Presentation
      常翔学園大阪センター
    • Year and Date
      2013-10-19
  • [Presentation] 発酵食品中のD-アミノ酸分析とその由来2013

    • Author(s)
      大森勇門、牟田口祐太、守﨑香菜、土居克実、大島敏久
    • Organizer
      日本生物高分子学会2013年度大会
    • Place of Presentation
      常翔学園大阪センター
    • Year and Date
      2013-10-19
  • [Presentation] 三者接合法によるGeobacillus kaustphilus HTA426への高効率プラスミド導入2013

    • Author(s)
      鈴木宏和、和田圭介、古川恵、土居克実、大島敏久
    • Organizer
      日本生物工学会第65回大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2013-09-18
  • [Presentation] 藍甕由来好アルカリ性Bacillus属細菌のインジゴカルミン還元酵素の機能解析2013

    • Author(s)
      安部友昭、鈴木宏昭、土居克実、大島敏久
    • Organizer
      日本農芸化学会関西・中四国・西日本支部、日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会2013年度合同広島大会
    • Place of Presentation
      広島県立大学
    • Year and Date
      2013-09-06
  • [Presentation] 小浜温泉より単離したファージφOH2由来耐熱性溶菌酵素の特性解析2013

    • Author(s)
      Martono Hindra、永吉佑子、藤野泰寛、大島敏久、土居克実
    • Organizer
      第50回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2013-07-06
  • [Presentation] 地熱環境より分離したThermus属繊維状ファージの性状解析2012

    • Author(s)
      熊谷健太、永吉佑子、藤野泰寛、大島敏久、土居克実
    • Organizer
      日本生物工学会九州支部大会
    • Place of Presentation
      別府大学
    • Year and Date
      2012-12-01
  • [Presentation] 色素依存性L-プロリン脱水素酵素を用いたL-プロリン電池の開発2012

    • Author(s)
      吉国翔一、若松泰介、土居克実、松本広重、大島敏久
    • Organizer
      日本生物工学会九州支部大会
    • Place of Presentation
      別府大学
    • Year and Date
      2012-12-01
  • [Presentation] サイレージ乳酸菌Pediococcus lolii NGRI 0510Q^Tのゲノム構造解析2012

    • Author(s)
      土居克実、森一樹、田代康介、藤野泰寛、久原哲、大島敏久
    • Organizer
      日本生物工学会2012年度大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
  • [Book] 地熱発電所と微生物、「環境と微生物」(日本微生物生態学会編)2014

    • Author(s)
      大島敏久、土居克実
    • Total Pages
      217-218
  • [Book] Biomineralization in geothermal environment, In Thermophilic Microbes in Environmental and Industrial Biotechnology(edited by T. Satyanarayana, J. Littlechild and Y. Kawarabayasi)2013

    • Author(s)
      K. Doi and Y. Fujino
    • Total Pages
      233-247
    • Publisher
      Springer, NY
  • [Remarks]

    • URL

      http://www.grt.kyushu-u.ac.jp/grt-docs/migt/HP/

URL: 

Published: 2015-06-25  

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