2013 Fiscal Year Final Research Report
Construction of novel molecular mechanism on polysaccharide-forming enzyme and its application
| Project/Area Number |
24658089
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
KIMURA Atsuo 北海道大学, (連合)農学研究科(研究院), 教授 (90186312)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 多糖 / ドメイン / 合成 |
|---|
| Research Abstract |
Purpose of this research is the elucidation of structural elements of polysaccharide-producing enzyme and middle-size-oligosaccharide-producing enzyme. Furthermore, the formation of new oligosaccharide is also purpose of this study by controlling the structural element. The obtained results were as follows; (1) Polysaccharide-producing enzyme: Catalytic amino acids were two Glu. Polysaccharide-forming structural element was in the C-terminal region of enzyme. (2) Middle-size-oligosaccharide-producing enzyme: Three-dimensional structure was elucidated, and catalytic residues were Asp and Glu. Mutation analyses of two aromatic residues at sites distant from catalytic center revealed that one residue improved the enzyme function.
|
