2013 Fiscal Year Final Research Report
Studies of factors that inhibit the macrophage migration
| Project/Area Number |
24658094
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
KIOKA Noriyuki 京都大学, (連合)農学研究科(研究院), 准教授 (90234179)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | メタボリック症候群 / 糖尿病 / マクロファージ / 細胞遊走 / 細胞接着 |
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| Research Abstract |
Macrophage invasion into adipose tissues plays a critical role for metabolic syndrome. An adaptor protein, CAP, is involved in the invasion of macrophages. Here we established the assay system for CAP-dependent macrophage migration. First we determined the optimum condition for examining macrophage migration. Then, expression of CAP was depleted in macrophage-like cells by shRNA for CAP. We found that CAP depletion promoted the macrophage migration estimated by xCELLigence. Re-expression of CAP isolated from macrophages rescued the migration. This system can be used for investigation of CAP-dependent macrophage migration.
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[Journal Article] Interaction of the vinculin proline-rich linker region with vinexin alpha in sensing extracellular matrix stiffness2014
Author(s)
Yamashita, H., Ichikawa, T., Matsuyama, D., Kimura, Y., Ueda, K., Craig, S. W., Harada, I. and Kioka, N.
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Journal Title
Journal of cell science
Volume: (In press)
DOI
Peer Reviewed
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[Journal Article] Vinexin-beta protects against cardiac hypertrophy by blocking the Akt-dependent signalling pathway2013
Author(s)
Chen, K., Gao, L., Liu, Y., Zhang, Y., Jiang, D. S., Wei, X., Zhu, X. H., Zhang, R., Chen, Y., Yang, Q. Kioka, N., Zhang, X. D., and Li, H.
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Journal Title
Basic research in cardiology
Volume: 108
Pages: 338
DOI
Peer Reviewed
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[Journal Article] Role of Dlg5/lp-dlg, a membrane-associated guanylate kinase family protein, in epithelial-mesenchymal transition in LLc-PK1 renal epithelial cells2012
Author(s)
Sezaki, T., Inada, K., Sogabe, T., Kakuda, K., Tomiyama, L., Matsuno, Y., Ichikawa, T., Matsuo, M., Ueda, K. and Kioka, N.
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Journal Title
PLOS One
Volume: 7
Pages: e35519
DOI
Peer Reviewed
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