Development of an efficient protein production system of various virus antigens for diagnostic and vaccine reagents in yeast

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Yamaguchi University
• Principal Investigator: AKADA Rinji 山口大学, 医学(系)研究科(研究院), 教授 (20201882)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ウイルス表面タンパク質 / 抗原 / HBsL / Saccharomyces cerevisiae / Kluyveromyces marxianus / 非相同末端結合 / ワクチン / 診断薬

Outline of Final Research Achievements

The recombinant virus proteins are used for vaccinations and diagnostic reagent kits and their low cost productions are required. In this study, efficient virus protein production system was developed in yeast. When hepatitis B virus surface protein HBsL was expressed in yeast, it was degraded rapidly. We constructed highly stable HBsL mutants and also an expression system that enable stable virus protein production in yeast. These results provide strategies for the problems of virus infection that will emerge in the future.

Free Research Field

遺伝子工学