Construction of high lipid content rice by the molecular mechanism analysis of the lipid synthesis in rice seed

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658097
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyoto Prefectural University
• Principal Investigator: TAKEHIRO Masumura 京都府立大学, 生命環境科学研究科(系), 講師 (50254321)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物 / 遺伝子発現 / 脂質 / イネ種子 / アリューロン層 / 転写因子 / 脂質合成遺伝子 / 形質転換イネ

Outline of Final Research Achievements

This study was intended to strengthen the aleurone layer which accumulated functional materials in rice seeds. High-coverage profiling analysis of gene expression in the aleurone layer was performed, and searched for important transcription factors or enzyme genes from the profiling data. The candidate clone was introduced into rice genome　to create high content of lipid accumulation in transgenic rice seed.
Under ubiquitin promoter control, the transgenic rice which overexpressed the transcription factor (NFYB) gene which specifically expressed during early differentiation stage of endosperm tissue was created. The seed morphology of the transgenic rice was observed, and lipid content was measured. The starchy endosperm tissues of transgenic seed were reduced, and the lipid contents per seed were increased. We found possibility to improve the lipid synthesis ability by decreasing the site of storage protein synthesis in transgenic seed by this study.

Free Research Field

植物分子生物学