2012 Fiscal Year Final Research Report

Identification of target proteins for ubiquitination in brown adipose tissue of fasted rat

Research Project

Project/Area Number	24658119
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	The University of Tokyo
Principal Investigator	NAKAI Yuji 東京大学, 大学院・農学生命科学研究科, 特任准教授 (10321788)
Project Period (FY)	2012
Keywords	ユビキチン / エネルギー代謝 / プロテアソーム阻害剤 / ラット / 褐色脂肪組織
Research Abstract	To clarify the mechanism for energy metabolism regulation by specific protein degradation, we attempt to identify the proteins that ubiquitinated in response to energy level in rat brown adipose tissue. Male Wistar rats aged 7 weeks old were subjected to 1 week restricted feeding, and then divided into 2 groups, fed and fasted. The rats were administrated epoxomicin, a potent proteasome inhibitor. The dose and method for administration of epoxomicin were determined to detect the difference of ubiquitinated protein levels between the 2 groups.

(1 results)