2012 Fiscal Year Final Research Report
Identification of target proteins for ubiquitination in brown adipose tissue of fasted rat
| Project/Area Number |
24658119
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAI Yuji 東京大学, 大学院・農学生命科学研究科, 特任准教授 (10321788)
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| Project Period (FY) |
2012
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| Keywords | ユビキチン / エネルギー代謝 / プロテアソーム阻害剤 / ラット / 褐色脂肪組織 |
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| Research Abstract |
To clarify the mechanism for energy metabolism regulation by specific protein degradation, we attempt to identify the proteins that ubiquitinated in response to energy level in rat brown adipose tissue. Male Wistar rats aged 7 weeks old were subjected to 1 week restricted feeding, and then divided into 2 groups, fed and fasted. The rats were administrated epoxomicin, a potent proteasome inhibitor. The dose and method for administration of epoxomicin were determined to detect the difference of ubiquitinated protein levels between the 2 groups.
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