2013 Fiscal Year Final Research Report
Investigation of target proteins by isothiocyanate with fluorescent probe
| Project/Area Number |
24658127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Food science
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| Research Institution | University of Hyogo |
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Principal Investigator |
KATO Yoji 兵庫県立大学, 環境人間学部, 教授 (30305693)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | イソチオシアネート / 蛍光 / アミノ付加体 |
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| Research Abstract |
Though isothiocyanates have some biological functions such as anti-cancer or anti-bacterial activities, the mechanisms has not been shown in detail. We found the covalent adduction of ITC with lysine in a protein at physiological conditions. In this study, we focused in fluorescent character of ITC by using FITC. A monoclonal antibody to fluorescent structure has been prepared and characterized and then applied to fluorescent staining of cells. The multiple ITC adducted proteins were then detected in a gel by fluorescent scanning or by specific antibody. The identification of ITC adducted proteins remains to be done.
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Research Products
(2 results)
