2012 Fiscal Year Final Research Report
A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing
| Project/Area Number |
24658138
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2012
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| Keywords | DNAマーカー / マイクロサテライト / 4塩基繰返し / 森林科学 / 育種学 / 分子生態学 |
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| Research Abstract |
DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.
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