Fish hematogenesis function associated with defense system to pathogens

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658176
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: KURATA Osamu 日本獣医生命科学大学, 獣医学部, 准教授 (90277666)
• Co-Investigator(Kenkyū-buntansha): NAKAYASU Chihaya 独立行政法人水産総合研究センター, その他 (00311225) ; OKAUCHI Masanori 独立行政法人水産総合研究センター, 研究員 (40372023) ; OZAKI Akiyuki 独立行政法人水産総合研究センター, 研究員 (40416045)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 造血細胞 / 移植 / 細胞分化 / 細胞表面マーカー / 遺伝子導入 / 魚類 / ヒラメ

Outline of Final Research Achievements

We aimed to analyze fish hematogenesis function by utilizing a hematopoietic precursor cell line established from Japanese flounder. We were successful in transplantation of the hematopoietic precursor cells (HPC) into fish with the same genetic lineage and larvae of flounder, Transplanted cells were detected in a hematopoietic organ (kidney) of recipient fish. Some monoclonal antibodies to cell surface molecules were established for estimation of differentiation in Japanese flounder hematopoietic cells. It was possible to recognize immature or mature cells using combinations of the antibodies. We tried to establish GFP-expressing HPC in order to track transplanted cells. Unfortunately, we could not achieve it but suitable expression vector and gene transfection method was found. As a new knowledge, cell division in the hematopoietic organ was induced 2 to 3 days after administration of pathogens.

Free Research Field

魚類免疫学