Snap-shot observation methods for natural aquatic microorganisms

2012 Fiscal Year Final Research Report

• Project/Area Number: 24658180
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Fisheries Research Agency
• Principal Investigator: NAGASAKI Keizo 独立行政法人水産総合研究センター, 研究推進部, 研究開発コーディネーター (00222175)
• Project Period (FY): 2012
• Keywords: 透過型電子顕微鏡 / 植物プランクトン / 凍結置換法 / 赤潮 / 微細藻類 / 水圏微生物生態学

Research Abstract

The objective of the present study is to establish a novel method for TEM observation of microalgae by using the “rapid freezing and freeze substitution” techniques. By using a portable deep freezer and half-frozen acetone, several strains of microalga were nicely prepared for TEM observation. The new technique is introduced at a website for its dissemination: (http://feis.fra.affrc.go.jp/keisou_Virus/kimura_freeze.html).

Research Products

• [Journal Article] Ultrastructural observation of natural field phytoplankton cells by using rapid freezing and freeze substitution (2012)
  • Author(s): Kei Kimura, Yuji Tomaru, Keizo Nagasaki
  • Journal Title: Plankton Benthos Res. Volume: 7(3) Pages: 126-134
  • Peer Reviewed
• [Presentation] Nano-sized wonder in red tides: surprised attack and peaceful coexistence (2012)
  • Author(s): Keizo Nagasaki
  • Organizer: Abstract book of Viruse of Microbes meeting III
  • Year and Date: 2012-07-18
• [Remarks]
  • URL: http://feis.fra.affrc.go.jp/keisou_Virus/kimura_freeze.html