2013 Fiscal Year Final Research Report
Research on the bivalve osmoregulatory system by transcriptome analysis
| Project/Area Number |
24658182
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
WATABE Shugo 北里大学, 海洋生命科学部, 教授 (40111489)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ヤマトシジミ / メタボローム解析 / トランスクリプトーム解析 / 塩分 / 涸沼 / 涸沼川 / 潮汐 / cDNAライブラリー |
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| Research Abstract |
The major fishing ground of the brackish water clam in Ibaraki Prefecture is located in the Hinuma Lake and Hinuma River. Water salinity in the Hinuma Lake is low and stable, whereas that in the downstream of the River varies daily. We collected the male and female clam from these areas and subjected to metabolome and transcriptome analyses. As a result, the concentrations of metabolites such as beta-alanine and ornithine were found to be changed in association with salinity. In addition, the levels of mRNAs encoding metabolic enzymes were also changed depending on salinity alteration. It is suggested that the brackish water clam changes a variety of metabolites in response to the alteration of environmental salinity, which is controlled by metabolite-related genes.
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