2013 Fiscal Year Final Research Report
Elusidation of microstructure of biofilm attached on the surface of agricultural produce
| Project/Area Number |
24658212
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Agricultural environmental engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
UCHINO Toshitaka 九州大学, (連合)農学研究科(研究院), 教授 (70134393)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Fumihiko 九州大学, 大学院農学研究院, 准教授 (30284912)
HAMANAKA Daisuke 九州大学, 大学院農学研究院, 助教 (60399095)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオフィルム / 共焦点レーザー顕微鏡 / 細胞外多糖類 / 乳製品副産物 / インデンテーション法 / 微細構造 |
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| Research Abstract |
An effect of dairy byproducts on adherence of dairy related bacteria to a stainless steel coupon surface conditioned by the byproducts was evaluated. The layer formed by skim milk buttermilk butter serum was thinnest, medium and thickest, respectively. Three dimensional images of biofilm were obtained by using a confocal laser scanning microscopy. In the result the conditioning by the byproducts controlled the bacterial adherence.
A hardness of biofilm was determined by indentation method. Twenty micro m of loading and 10 micro m of unloading by a universal material testing machine were repeated until a plunger head touching a plastic coupon. Thickness of biofilm was calculated from a length between contact points of the plunger head with the coupon and biofilm. The biofilm strength increased with decrease of biofilm thickness.
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