Cyclophilin A is a early stage maker of bovine mastitis.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658224
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Tohoku University
• Principal Investigator: ASO Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): KITAZAWA Haruki 東北大学, 大学院農学研究科, 准教授 (10204885) ; WATANABE Kouichi 東北大学, 大学院農学研究科, 助教 (80261494)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 乳房炎 / シクロフィリンA / 遊走因子 / 診断法 / 炎症 / 乳汁 / CyPA / 乳腺上皮細胞

Research Abstract

Cyclophilin A (CyPA), a member of the peptidyl-prolyl cis-trans isomerase family, is known as a cyclosporin binding-protein. Although CyPA was initially believed to function primarily as an intracellular protein, recent studies have revealed that it can be secreted by cells in response to inflammatory stimuli. Therefore, CyPA may play a role in cardiovascular diseases, viral infections and others. Further, extracellular CyPA has a potent chemotactic effect on leukocytes, monocytes, and lymphocytes through CD147 cell receptor.
We investigated the expression of CyPA in bovine mammary gland. CyPA was detected in mammary epithelial cells of mastitis cow stronger than healthy cow. Surprisingly, CyPA in milk was stained in mammary gland alveolus of mastitis cow with inflammation of leukocytes, but not in healthy cow. The content of CyPA in milk was greater in mastitis cow than healthy cow. These data indicate that CyPA in milk may be a marker of mastitis.

Research Products

• [Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double muscled (OM) and normal muscled (NM) Japanese shorthorn cattle (2014)
  • Author(s): H. Takahashi,K. Sato,T. Yamaguchi,M. Miyake,H. Watanabe,Y. Nagasawa,E. Kitagawa,S. Terada,M. Urakawa,M. T. Rose,C. O. McMahon,K. Watanabe,S. Ohwada,T. Gotoh,H. Aso
  • Journal Title: Oomestic Animal Endocrinology Volume: 48巻 Pages: 62-68
  • DOI: 10.1016/j.domaniend.2014.01.007
  • Peer Reviewed
• [Journal Article] Effect of peripheral 5-HT on glucose and lipid metabolism in wether sheep (2014)
  • Author(s): Hitoshi Watanabe,Ryo Saito,Tatsuya Nakano,Hideyuki Takahashi,Yu Takahashi,Keisuke Sumiyoshi,Katsuyoshi Sato,Xiangning Chen,Natsumi Okada,Shunsuke Iwasaki,Oian W. Harjanti,Natsumi Sekiguchi,Hiroaki Sano,Haruki Kitazawa,Michael T. Rose,Shyuichi Ohwada,Kouichi Watanabe,Hisashi Aso
  • Journal Title: PLoS ONE Volume: 9(2)巻 Pages: e88058
  • DOI: 10.1371/journal.pone.0088058
  • Peer Reviewed
• [Journal Article] Immunoregulatory effect of bifidobacteria strains in porcine intestinal epithelial cells through modulation of ubiquitin-editing enzyme A20 expression (2013)
  • Author(s): Tomosada,Y.,J. Villena,K. Murata,E. Chiba,T. Shimazu,H. Aso,N. Iwabuchi,J.-z. Xiao,T. Saito,H. Kitazawa
  • Journal Title: PLoS ONE Volume: 8(3)巻 Pages: e59259
  • DOI: 10.1371/journal.pone.0059259
  • Peer Reviewed
• [Journal Article] Bifidobacteria Upregulate Expression of Toll-Like Receptor Negative Regulators Counteracting Enterotoxigenic Escherichia coli Mediated Inflammation in Bovine Intestinal Epitheliocytes (2013)
  • Author(s): Kozue Murata,Julio Villena,Yohsuke Tomosada,Risa Hara,Eriko Chiba,Tomoyuki Shimazu,Hisashi Aso,Yoshihito Suda,Noriyuki Iwabuchi,Jin-Zhong Xiao,Tadao Saito,Haruki Kitazawa
  • Journal Title: Open Journal of Veterinary Medicine Volume: 3巻 Pages: 143-155
  • DOI: 10.4236/ojvm.2013.32023
  • Peer Reviewed
• [Journal Article] Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation (2013)
  • Author(s): Takanashi,N.,Y. Tomosada,J. Villena,K. Murata,T. Takahashi,E. Chiba,M. Tohno,T. Shimazu,H. Aso,Y. Suda,S. Ikegami,H. Itoh,Y. Kawai,T. Saito,S. Alvarez,H. Kitazawa
  • Journal Title: BMC Microbiology Volume: 13巻 Pages: 54-69
  • DOI: 10.1186/1471-2180-13-54
  • Peer Reviewed
• [Journal Article] Expression of myostatin in neural cells of the olfactory system (2013)
  • Author(s): Iwasaki S.,Miyake M.,Watanabe H.,Kitagawa E.,Watanabe K.,Ohwada S.,Kitazawa H.,Rose M.T.,Aso H.
  • Journal Title: Molecular Neurobiology Volume: 47巻 Pages: 1-8
  • DOI: 10.1007/s12035-012-8342-1
  • Peer Reviewed
• [Journal Article] AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle (2012)
  • Author(s): Miyake M,Takahashi H,Kitagawa E,Watanabe H,Sakurada T,Aso H,Yamaguchi T
  • Journal Title: Cell and Tissue Research Volume: 349(2)巻 Pages: 615-623
  • DOI: 10.1007/s00441-012-1422-8
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cellsJournal (2012)
  • Author(s): Villena,J.,R. Suzuki,H. Fujie,E. Chiba,T. Takahashi,Y. Tomosada,T. Shimazu,H. Aso,S. Ohwada,Y. Suda,S. Ikegami,H. Itoh,S. Alvarez,T. Saito,H. Kitazawa
  • Journal Title: Clinical and Vaccine Immunology Volume: 19(7)巻 Pages: 1038-1053
  • DOI: 10.1128/CVI.00199-12
  • Peer Reviewed
• [Journal Article] A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle (2012)
  • Author(s): Eriko Chiba,Julio Villena,Shoichi Hosoya,Naoya Takanashi,Tomoyuki Shimazu,Hisashi Aso,Masanori Tohno,Yoshihito Suda,Yasushi Kawai,Tadao Saito,Kenji Miyazawa,Fang He,Haruki Kitazawa
  • Journal Title: Research in Veterinary Science Volume: 93巻 Pages: 688-394
  • DOI: 10.1016/j.rvsc.2011.10.002
  • Peer Reviewed
• [Journal Article] Effects of a Lactobacilli,Oligosaccharide and Organic Germanium Intake on the Immune Responses of Mice (2012)
  • Author(s): Takashi NAKAMURA,Miki SAITO and Hisashi ASO
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 76 (2)巻 Pages: 375-377
  • DOI: 10.1271/bbb.110655
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway (2012)
  • Author(s): Tomoyuki Shimazu,Julio Villena,Masanori Tohno,Hitomi Fujie,Shoichi Hosoya,Takeshi Shimosato,Hisashi Aso,Yoshihito Suda,Yasushi Kawai,Tadao Saito,Seiya Makino,Shuji Ikegami,Hiroyuki Itoh,Haruki Kitazawa
  • Journal Title: Infection and Immunity Volume: 80 (1)巻 Pages: 276-288
  • DOI: 10.1128/IAI.05729-11
  • Peer Reviewed
• [Presentation] 乳房炎牛乳汁中に増量するシクロフィリンAの細胞遊走活性 (2014)
  • Author(s): 高梨暁
  • Organizer: 第118回日本畜産学会大会
  • Place of Presentation: つくば市、国際会館
  • Year and Date: 2014-03-27
• [Presentation] 乳房炎多発症牛への枯草菌給与における血中樹状細胞の動態 (2013)
  • Author(s): 浦川めぐみ
  • Organizer: 第117回日本畜産学会大会
  • Place of Presentation: 新潟市、新潟大学
  • Year and Date: 2013-09-09
• [Presentation] 乳房炎発症牛への枯草菌給与における栄養因子への影響 (2013)
  • Author(s): 佐藤勝祥
  • Organizer: 第117回日本畜産学会大会
  • Place of Presentation: 新潟市、新潟大学
  • Year and Date: 2013-09-09
• [Presentation] 乳房炎発症牛への枯草菌給与における分娩後の栄養因子およびストレス指標の変動 (2013)
  • Author(s): 佐藤勝祥
  • Organizer: 第63回東北畜産学会大会
  • Place of Presentation: 山形市、山形テルサ
  • Year and Date: 2013-08-29
• [Presentation] 乳房炎多発症牛への枯草菌給与による乳房炎発症予防と血中樹状細胞の動態 (2013)
  • Author(s): 浦川めぐみ
  • Organizer: 第63回東北畜産学会大会
  • Place of Presentation: 山形市、山形テルサ
  • Year and Date: 2013-08-29
• [Presentation] 腸管M細胞における解糖系酵素アルドラーゼAを介したプリオン蛋白質取り込み機構 (2013)
  • Author(s): 長津裕哉
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 名古屋、安田女子大学
  • Year and Date: 2013-03-28
• [Presentation] 炎症性腸疾患における腸上皮細胞由来シクロフィリンAの作用解明 (2013)
  • Author(s): 染谷俊輔
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 名古屋、安田女子大学
  • Year and Date: 2013-03-28
• [Presentation] Role of aldolase A in transcytosis of intestinal M cells (2012)
  • Author(s): Nagasawa Y.
  • Organizer: 2012 ASCB Annual Meeting. The American Society for Cell Biology
  • Place of Presentation: San Francisco,USA
  • Year and Date: 2012-12-17
• [Presentation] 乳汁中リンパ球遊走因子シクロフィリンAの乳房炎早期診断マーカーへの応用 (2012)
  • Author(s): 麻生久
  • Organizer: 第17回日本乳房炎研究会学術集会
  • Place of Presentation: 南青山会館、東京
  • Year and Date: 2012-10-12
• [Presentation] 腸管M細胞における解糖系酵素アルドラーゼAを介した抗原取り込み機構 (2012)
  • Author(s): 長津裕哉
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 2012-09-14
• [Presentation] マウスOSS誘導大腸炎と白血球遊走因子シクロフィリンAとの関連性 (2012)
  • Author(s): 染谷俊輔
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 2012-09-14
• [Presentation] 高抗病性ランドレース豚の免疫担当臓器におけるサイトカイン発現の特性解析 (2012)
  • Author(s): 菅原静夏
  • Organizer: 第62回東北畜産学会大会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2012-08-29
• [Presentation] Prion protein binding proteins of bovine intestine M cell (2012)
  • Author(s): Nagasawa Y.
  • Organizer: Asian Pacific Prion Symposium 2012
  • Place of Presentation: Yokohama,Japan
  • Year and Date: 2012-07-29
• [Book] 乳房炎の防除:乳質向上と免疫力アップ (2012)
  • Author(s): 麻生久
  • Total Pages: 175
  • Publisher: デーリー・ジャパン社
• [Book] The Expression of Cytokeratins in Bovine Intestinal Microfold (M) Cells.(Cytokeratins-Tools in Oncology,Gerhard Hamilton (Ed.)) (2012)
  • Author(s): Takashi Kanaya,Tetsuya Hondo,Kohtaro Miyazawa,Michael T. Rose and Hisashi Aso
  • Total Pages: 3-14
  • Publisher: InTech
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/keitai/index-j.html