Studies on ammonia exposure to trichothecene mycotoxin, deoxynivalenol

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658226
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Mie University
• Principal Investigator: KONDO Makoto 三重大学, 生物資源学研究科, 助教 (50432175)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: デオキシニバレノール / アンモニア / 細胞毒性

Research Abstract

Research was conducted to determine the effect of ammonia exposure to trichothecene mycotoxin, deoxynivalenol (DON). DON concentration decreased by ammonia exposure in a dose dependent manner. DON was transformed both reversibly and irrevesibly by a short period (24 h) of ammoniation. In contrast, after 3 weeks ammoniation, DON was not detected completely. Cytotoxicity to human leukemia cell line HL60 by DON-NH3 reacted solution was present after 24 h reaction, but not detected any toxicity after 3 week reaction.