2013 Fiscal Year Final Research Report
Promotion of development of in vitro produced bovine embryos by using emulsified lipophilic nutrients
| Project/Area Number |
24658233
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
IKEDA Shuntaro 京都大学, (連合)農学研究科(研究院), 助教 (50447893)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ウシ / 受精卵 / 初期胚 / 体外受精 / 脂質 / 乳化 |
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| Research Abstract |
We developed a simple and stable method for emulsification of bioactive lipophilic nutrients intended to add them into culture media of in vitro produced bovine embryos. Emulsified mixture of alpha-tocopherol and beta-carotene (emVEBC) enhanced hatching rate of blastocysts, whereas emulsified oleic acid decreased blastocyst development. To elucidate mechanisms of the favorable effects of emVEBC on embryonic development, a microarray-based transcriptomic analysis of blastocysts was implemented and candidates of responsible genes were listed.
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