2013 Fiscal Year Final Research Report

Involvement of maternal mRNA in the regulation of differentiation during preimplantation development.

Research Project

Project/Area Number	24658244
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	The University of Tokyo
Principal Investigator	AOKI Fugaku 東京大学, 新領域創成科学研究科, 教授 (20175160)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	初期発生 / 細胞分化 / 母性mRNA / 2細胞期胚 / 4細胞期胚
Research Abstract	To identify the maternal mRNA that are involved in the differentiation during preimplantation development, I conducted RNA sequencing analysis using the oocytes and embryos. The results showed that although 70% of maternal mRNAs were degraded soon after fertilization, the rest of them survived this wave of a rapid degradation. Especially, those from 691 genes were hardly degraded during the 1-cell stage. Gene Ontology (GO) enrichment analysis revealed that these genes were enriched in the GO terms categorized into transcription.

(2 results)