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2013 Fiscal Year Final Research Report

Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection

Research Project

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Project/Area Number 24658245
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

OZAWA Makoto  鹿児島大学, 獣医学部, 准教授 (50568722)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsインフルエンザウイルス / トランスジェニックマウス / レポーター遺伝子
Research Abstract

To generate transgenic mouse ubiquitously expressing a recombinant influenza virus-like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.

  • Research Products

    (28 results)

All 2014 2013 2012

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus2014

    • Author(s)
      Fonseca W, Ozawa M, Hatta M, Orozco E, Martinez MB, Kawaoka Y
    • Journal Title

      Arch Virol

      Volume: 159 Pages: 1067-1077

    • DOI

      10.1007/978-1-61779-621-0_12

    • Peer Reviewed
  • [Journal Article] B-Cell-Intrinsic Hepatitis C Virus Expression Leads to B-Cell-Lymphomagenesis and Induction of NF-kappaB Signalling2014

    • Author(s)
      Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T, Tsukiyama-Kohara K
    • Journal Title

      PLoS One

      Volume: 9 Pages: e91373

    • DOI

      10.1371/journal.pone.0091373

    • Peer Reviewed
  • [Journal Article] Recent insights into hepatitis B virus-host interactions2014

    • Author(s)
      Ezzikouri S, Ozawa M, Kohara M, Elmdaghri N, Benjelloun S, Tsukiyama-Kohara K
    • Journal Title

      J Med Virol

      Volume: 86 Pages: 925-932

    • DOI

      10.1002/jmv.23916

    • Peer Reviewed
  • [Journal Article] Characterization of H7N9 influenza A viruses isolated from humans2013

    • Author(s)
      Watanabe T, Kiso M, Fukuyama S, Nakajima N, Imai M, Yamada S, Murakami S, Yamayoshi S, Iwatsuki-Horimoto K, Sakoda Y, Takashita E, McBride R, Noda T, Hatta M, Imai H, Zhao D, Kishida N, Shirakura M, de Vries RP, Shichinohe S, Okamatsu M, Tamura T, Tomita Y, Fujimoto N, Goto K, Katsura H, Kawakami E, Ishikawa I, Watanabe S, Ito M, Sakai-Tagawa Y, Sugita Y, Uraki R, Yamaji R, Eisfeld AJ, Zhong G, Fan S, Ping J, Maher EA, Hanson A, Uchida Y, Saito T, Ozawa M, Neumann G, Kida H, Odagiri T, Paulson JC, Hasegawa H, Tashiro M, Kawaoka Y
    • Journal Title

      Nature

      Volume: 501 Pages: 551-555

    • DOI

      10.1038/nature12392

    • Peer Reviewed
  • [Journal Article] Bivalent Vaccine Based on a PB2-Knockout Influenza Virus Protects Mice from Pandemic H1N1 and Highly Pathogenic H5N1 Virus Challenges2013

    • Author(s)
      Uraki R, Kiso M, Iwatsuki-Horimoto K, Fukuyama S, Takashita E, Ozawa M, Kawaoka Y. A Novel
    • Journal Title

      J Virol

      Volume: 87 Pages: 7874-7881

    • DOI

      10.1128/JVI.00076-13

    • Peer Reviewed
  • [Journal Article] Synergistic Effect of the PDZ and p85beta-Binding Domains of the NS1 Protein on Virulence of an Avian H5N1 Influenza A Virus2013

    • Author(s)
      Fan S, Macken CA, Li C, Ozawa M, Goto H, Iswahyudi NF, Nidom CA, Chen H, Neumann G, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 87 Pages: 4861-4871

    • DOI

      10.1128/JVI.02608-12

    • Peer Reviewed
  • [Journal Article] A cell-based screening system for influenza A viral RNA transcription/replication inhibitors2013

    • Author(s)
      Ozawa M, Shimojima M, Goto H, Watanabe S, Hatta Y, Kiso M, Furuta Y, Horimoto T, Peters NR, Hoffmann FM, Kawaoka Y
    • Journal Title

      Sci Rep

      Volume: 3 Pages: 1106

    • DOI

      10.1038/srep01106

    • Peer Reviewed
  • [Journal Article] Genomic polymorphisms in 3beta-hydroxysterol Delta24-reductase promoter sequences2013

    • Author(s)
      Salem NE, Saito M, Kasama Y, Ozawa M, Kawabata T, Harada S, Suda H, Asonuma K, El-Gohary A, Tsukiyama-Kohara K
    • Journal Title

      Microbiol Immunol

      Volume: 57 Pages: 179-184

    • DOI

      10.1111/1348-0421

    • Peer Reviewed
  • [Journal Article] Intrinsic temperature sensitivity of influenza C virus hemagglutinin-esterase-fusion protein2012

    • Author(s)
      Takashita E, Muraki Y, Sugawara K, Asao H, Nishimura H, Suzuki K, Tsuji T, Hongo S, Ohara Y, Kawaoka Y, Ozawa M, Matsuzaki Y
    • Journal Title

      J Virol

      Volume: 86 Pages: 13108-13111

    • DOI

      10.1128/JVI.01925-12

    • Peer Reviewed
  • [Journal Article] Competitive incorporation of homologous gene segments of influenza A virus into virions2012

    • Author(s)
      Inagaki A, Goto H, Kakugawa S, Ozawa M, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 86 Pages: 10200-10202

    • DOI

      10.1128/JVI.01204-12

    • Peer Reviewed
  • [Journal Article] Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets2012

    • Author(s)
      Imai M, Watanabe T, Hatta M, Das SC, Ozawa M, Shinya K, Zhong G, Hanson A, Katsura H, Watanabe S, Li C, Kawakami E, Yamada S, Kiso M, Suzuki Y, Maher EA, Neumann G, Kawaoka Y
    • Journal Title

      Nature

      Volume: 486 Pages: 420-428

    • DOI

      10.1038/nature10831

    • Peer Reviewed
  • [Journal Article] Characterization in vitro and in vivo of pandemic (H1N1) 2009 influenza viruses isolated from patients2012

    • Author(s)
      Watanabe T, Imai M, Watanabe S, Shinya K, Hatta M, Li C, Neumann G, Ozawa M, Hanson A, Zhong G, Fukuyama S, Kawakami E, Simmons HA, Schenkman D, Brunner K, Capuano SV, 3rd, Weinfurter JT, Kilander A, Dudman SG, Suresh M, Hungnes O, Friedrich TC, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 86 Pages: 9361-9368

    • DOI

      10.1128/JVI.01214-12

    • Peer Reviewed
  • [Journal Article] Reverse genetics of influenza viruses2012

    • Author(s)
      Neumann G, Ozawa M, Kawaoka Y
    • Journal Title

      Methods Mol Biol

      Volume: 865 Pages: 193-206

    • DOI

      10.1007/978-1-61779-621-0_12

  • [Presentation] ツル越冬地の水からのインフルエンザウイルスの分離2013

    • Author(s)
      小澤真、奥谷公亮、川畑淑子、永野希織、楠元勇、高瀬公三、小原恭子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      20131100
  • [Presentation] Development and Aapplication of replication-incompetent foreign gene-expressing influenza viruses2013

    • Author(s)
      Makoto Ozawa and Yoshihiro Kawaoka
    • Organizer
      International Symposium of Korea Research Institute of Bioscience and Biotechnology
    • Place of Presentation
      Hwaseong (Republic of Korea)
    • Year and Date
      20131000
  • [Presentation] 口蹄疫ウイルスのIRES依存性翻訳活性の評価2013

    • Author(s)
      神田雄大、小澤真、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      20130900
  • [Presentation] ツパイとヒトとの遺伝的相同性の検証2013

    • Author(s)
      金澤伯弘、川畑淑子、永野希織、小原道法、小澤真、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      20130900
  • [Presentation] ツルのねぐらにおけるインフルエンザウイルスの分離2013

    • Author(s)
      奥谷公亮、川畑淑子、永野希織、楠元勇、高瀬公三、小原恭子、小澤真
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      20130900
  • [Presentation] 養豚における抗インフルエンザウイルス抗体の保有状況2013

    • Author(s)
      小澤真、川畑淑子、出口栄三郎、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      20130900
  • [Presentation] Influenza viral hemagglutinin-targeted macrocycles as an antiviral agent2013

    • Author(s)
      M. Saito, M. Ozawa, F. Yasui, T. Sasaki, T. Munakata, Y. Tobita, R. Ito, K. Munekata, K. Tsukiyama-Kohara, A. Sakurai, F. Shibasaki, Y. Sakoda, H. Kida, P. C. Reid, K. Kubota, H. Suga, Kohara
    • Organizer
      Options for the Control of Influenza VIII
    • Place of Presentation
      Cape Town (South Africa)
    • Year and Date
      20130900
  • [Presentation] A cell-based screening system for influenza A viral RNA transcription/replication inhibitors2013

    • Author(s)
      M. Ozawa, M. Shimojima, H. Goto, S. Watanabe, Y. Hatta, M. Kiso, Y. Furuta, T. Horimoto, R. Peters, F. M. Hoffmann, Y. Kawaoka
    • Organizer
      15th International Negative Strand Virus Meeting
    • Place of Presentation
      Granada (Spain)
    • Year and Date
      20130700
  • [Presentation] Development and Aapplication of replication-incompetent foreign gene-expressing influenza viruses2013

    • Author(s)
      Makoto Ozawa and Yoshihiro Kawaoka
    • Organizer
      International Symposium of Tokyo Metropolitan Institute of Medical Sccience
    • Place of Presentation
      Tokyo (Japan)
    • Year and Date
      20130200
  • [Presentation] 豚インフルエンザの現状2013

    • Author(s)
      小澤真
    • Organizer
      鹿児島県養豚研究会平成24年度第2回研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      2013-01-23
  • [Presentation] 生体内ライブイメージングを可能とする組換えA型インフルエンザウイルスの作出2012

    • Author(s)
      小澤真、福山聡、桂廣亮、石川いずみ、河岡義裕
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20121100
  • [Presentation] 非増殖性・外来遺伝子発現組換えインフルエンザウイルスの作出とワクチン効果の検討2012

    • Author(s)
      小澤真、Sylvia Victor、渡辺真治、角川学士、桂廣亮、河岡義裕
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Year and Date
      20120900
  • [Presentation] 豚におけるインフルエンザについて2012

    • Author(s)
      小澤真
    • Organizer
      南九州養豚会第111回定例会
    • Place of Presentation
      鹿児島
    • Year and Date
      20120700
  • [Book] 専門家に聞くインフルエンザウイルス講座「インフルエンザウイルスの分類法―科・型・亜型―」インフルエンザ15(1)2014

    • Author(s)
      小澤真、河岡義裕
    • Total Pages
      45-47
  • [Patent(Industrial Property Rights)] Influenza viruses with mutant PB2 gene segment as live attenuated vaccines2012

    • Inventor(s)
      Yoshihiro Kawaoka, Gabriele Neumann, Makoto Ozawa
    • Industrial Property Rights Holder
      Yoshihiro Kawaoka, Gabriele Neumann, Makoto Ozawa
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      Ref : P110003US02, App.#: 13/594611
    • Acquisition Date
      2012-08-24
    • Overseas

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Published: 2015-06-25  

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