Studies on mechanisms of heme-sewage managements in the tick midgut

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658251
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: NAOTOSHI Tsuji 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・動物疾病対策センター生物学的製剤製造グループ, グループ長 (70355171)
• Co-Investigator(Renkei-kenkyūsha): HATTA Takeshi 独立行政法人農業・食品産業技術総合研究機構・動物衛生研究所, 細菌・寄生虫研究領域, 主任研究員 (00455304)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 獣医学 / 感染症 / 吸血性節足動物 / マダニ / ヘモグロビン / ヘム

Research Abstract

Ticks employ a battery of proteases to digest the contents of host blood meals. Host hemoglobin degradation is facilitated by proteolytic networks in the midgut, the first major region of the body where ingested blood comes in to contact with the tick's internal tissues. The present study revealed existence of hemoglobin degradation pathway which consists of proteolytic enzymes and their inhibitors within the midgut epithelium. In particular, expression of a cathepsin L-like cysteine protease, isolated from the ixodid tick Haemaphysalis longicornis (HlCPL-A) is correlated with Hb digestion. Up and down-regulation of heme-sewage metabolism is essential for the survival of ticks, since blood meals from hosts are the only source of energy, suggesting that this pathway may be suitable drug targets for better control of ticks and tick-borne diseases.

Research Products

• [Journal Article] Two kinds of ferritin protect ixodid ticks from iron overload and consequent oxidative stress (2014)
  • Author(s): Galay RL, Umemiya-Shirafuji R, Bacolod ET, Maeda H, Kusakisako K, Koyama J, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T
  • Journal Title: PLoS One Volume: 9(3) Pages: e90661
  • DOI: 10.1371/journal.pone.0090661
  • Peer Reviewed
• [Journal Article] HlCPL-A, a cathepsin L-like cysteine protease from the ixodid tick Haemaphysalis longicornis, modulated midgut proteolytic enzymes and their inhibitors during blood meal digestion (2013)
  • Author(s): Yamaji K, Miyoshi T, Hatta T, Matsubayashi M, Alim MA, Anisuzzaman, Kushibiki S, Fujisaki K, Tsuji N
  • Journal Title: Infect Genet Evol Volume: 16c Pages: 206-211
  • DOI: 10.1016/j.meegid.2013.01.018
  • Peer Reviewed
• [Presentation] マダニの吸血生理 (2014)
  • Author(s): 辻尚利
  • Organizer: 平成25年度日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2014-02-21
• [Presentation] マダニアレイ解析によるLeucine rich-repeat (LRR) protein-homolog遺伝子の検出 (2013)
  • Author(s): 八田岳士,松林誠,三好猛晴,アニスザマン,アリムアブドゥル,藤崎幸蔵,辻尚利
  • Organizer: 第11回分子寄生虫・マラリアフォーラム
  • Place of Presentation: 長崎熱帯医学研究所(長崎市)
  • Year and Date: 2013-10-02
• [Presentation] Longistatin efficiently modulates inflammation during tick's feeding by antagonizing RAGE, a multi-ligand receptor (2013)
  • Author(s): Anisuzzaman,三好猛晴,八田岳士,松林誠,Khyrul Islam,Abdul Alim,藤崎幸蔵,辻尚利
  • Organizer: 第11回分子寄生虫・マラリアフォーラム
  • Place of Presentation: 長崎熱帯医学研究所(長崎市)
  • Year and Date: 2013-10-02
• [Presentation] マダニロンギスタチンのRAGE吸着を介した宿主炎症反応の抑制 (2013)
  • Author(s): アニスザマン,三好猛晴,八田岳士,松林誠,イスラムカイルル,アリムアブドゥル,藤崎幸蔵,辻尚利
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学湯島キャンパス(文京区)
  • Year and Date: 2013-03-29
• [Presentation] 人工吸血法により作出したバベシア原虫感染マダニの中腸mRNA-seq解析 (2012)
  • Author(s): 八田岳士,三好猛晴,松林誠,アニスザマン,アリムアブドゥル,山地佳代子,五十嵐郁男,藤崎幸蔵,辻尚利
  • Organizer: 第10回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 群馬大学医学部(前橋市)
  • Year and Date: 2012-10-02
• [Remarks]
  • URL: http://www.niah.affrc.go.jp/collab/person/tsuji/index.html